发明授权
US5750372A Vector having promoter that is inducible by methanol and/or glycerol
失效
具有可被甲醇和/或甘油诱导的启动子的载体
- 专利标题: Vector having promoter that is inducible by methanol and/or glycerol
- 专利标题(中): 具有可被甲醇和/或甘油诱导的启动子的载体
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申请号: US485284申请日: 1995-06-07
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公开(公告)号: US5750372A公开(公告)日: 1998-05-12
- 发明人: Yasuyoshi Sakai , Yoshiki Tani , Yuji Shibano , Hiroto Kondo , Haruyo Hatanaka
- 申请人: Yasuyoshi Sakai , Yoshiki Tani , Yuji Shibano , Hiroto Kondo , Haruyo Hatanaka
- 申请人地址: JPX Osaka
- 专利权人: Suntory Limited
- 当前专利权人: Suntory Limited
- 当前专利权人地址: JPX Osaka
- 优先权: JPX4-043361 19920228
- 主分类号: C12N1/19
- IPC分类号: C12N1/19 ; C12N9/00 ; C12N9/02 ; C12N9/04 ; C12N9/08 ; C12N9/12 ; C12N15/09 ; C12N15/11 ; C12N15/53 ; C12N15/54 ; C12N15/81 ; C12P21/00 ; C12R1/01 ; C12R1/72 ; C12R1/865 ; C12P21/06
摘要:
The present invention relates to an expression vector which is induced by methanol and/or glycerol utilizing an alcohol oxidase gene of a methylotrophic yeast; a recombinant methylotrophic yeast containing said vector and capable of accumulating a significantly high amount of an expression product of a heterologous gene; and a method for producing useful products utilizing said recombinant. The expression vector is constructed by utilizing the promoter and terminator of an alcohol oxidase gene of methylotrophic yeast. Further, a significantly high amount of adenylate kinase, cytochrome C552 or peroxidase is produced by using said expression vector.
公开/授权文献
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