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US5750372A Vector having promoter that is inducible by methanol and/or glycerol 失效
具有可被甲醇和/或甘油诱导的启动子的载体

Vector having promoter that is inducible by methanol and/or glycerol
摘要:
The present invention relates to an expression vector which is induced by methanol and/or glycerol utilizing an alcohol oxidase gene of a methylotrophic yeast; a recombinant methylotrophic yeast containing said vector and capable of accumulating a significantly high amount of an expression product of a heterologous gene; and a method for producing useful products utilizing said recombinant. The expression vector is constructed by utilizing the promoter and terminator of an alcohol oxidase gene of methylotrophic yeast. Further, a significantly high amount of adenylate kinase, cytochrome C552 or peroxidase is produced by using said expression vector.
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