发明授权
- 专利标题： D29 shuttle phasmids and uses thereof
- 专利标题（中）： D29穿梭质粒及其用途
-
申请号： US614770申请日： 1996-03-07
-
公开(公告)号： US5773267A公开(公告)日： 1998-06-30
- 发明人: William R. Jacobs , Graham F. Hatfull
- 申请人： William R. Jacobs , Graham F. Hatfull
- 申请人地址： NY Bronx PA Pittsburgh
- 专利权人： Albert Einstein College of Medicine of Yeshiva University, a Division of Yeshiva University,University of Pittsburgh
- 当前专利权人： Albert Einstein College of Medicine of Yeshiva University, a Division of Yeshiva University,University of Pittsburgh
- 当前专利权人地址： NY Bronx PA Pittsburgh
- 主分类号： C07K14/01
- IPC分类号： C07K14/01 ; C07K14/35 ; C12N1/21 ; C12N7/00 ; C12N15/74 ; C12Q1/68 ; C12N15/11 ; C12N15/63
摘要：
The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the D29 mycobacteriophage which is capable of introducing DNA of interest into the chromosome of mycobacteria, especially M. tuberculosis complex organisms and other slow growing mycobacteria. The present invention provides a recombinant mycobacterium which expresses a DNA of interest incorporated into its chromosome by a conditional shuttle plasmid containing the DNA of interest. The present invention further provides a mycobacterial auxotrophic mutant and method of generating auxotrophic mutants. Finally, the present invention provides a method of inactivating a mycobacterial virulence gene.
公开/授权文献
- US5234858A Stacked surrounding wall capacitor 公开/授权日：1993-08-10
信息查询
