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公开(公告)号：US5773267A
公开(公告)日：1998-06-30
申请号：US614770
申请日：1996-03-07
CPC分类号： C07K14/005 , C07K14/35 , C12N15/74 , C12N7/00 , C12Q1/6897 , C12N2795/10311 , C12N2795/10322 , C12N2795/10343
摘要： The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the D29 mycobacteriophage which is capable of introducing DNA of interest into the chromosome of mycobacteria, especially M. tuberculosis complex organisms and other slow growing mycobacteria. The present invention provides a recombinant mycobacterium which expresses a DNA of interest incorporated into its chromosome by a conditional shuttle plasmid containing the DNA of interest. The present invention further provides a mycobacterial auxotrophic mutant and method of generating auxotrophic mutants. Finally, the present invention provides a method of inactivating a mycobacterial virulence gene.
摘要翻译： 本发明提供了一种通过将粘粒插入D29分枝杆菌噬菌体的非必需区域而构建的条件穿梭质粒，其能够将感兴趣的DNA引入分枝杆菌染色体，特别是结核分枝杆菌复合生物体和其它缓慢生长的分枝杆菌。 本发明提供了一种重组分枝杆菌，其通过含有感兴趣的DNA的条件穿梭质粒表达目的DNA掺入其染色体中。 本发明还提供了分枝杆菌营养缺陷突变体和产生营养缺陷突变体的方法。 最后，本发明提供灭活分枝杆菌毒力基因的方法。
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公开(公告)号：US06300061B1
公开(公告)日：2001-10-09
申请号：US08705557
申请日：1996-08-29
IPC分类号： C12Q168
CPC分类号： C07K14/005 , C07K14/35 , C12N7/00 , C12N15/74 , C12N2795/10322 , C12Q1/18 , C12Q1/6897 , G01N33/5695
摘要： This invention relates to mycobacterial species-specific reporter mycobacteriophages (reporter mycobacteriophages), methods of producing said reporter mycobacteriophages and the use of said reporter mycobacteriophages for the rapid diagnosis of mycobacterial infection and the assessment of drug susceptibilities of mycobacterial strains in clinical samples. In particular, this invention is directed to the production and use of luciferase reporter mycobacteriophages to diagnose tuberculosis. The mycobacterial species-specific reporter mycobacteriophages comprise mycobacterial species-specific mycobacteriophages which contain reporter genes and transcriptional promoters therein. When the reporter mycobacteriophages are incubated with clinical samples which may contain the mycobacteria of interest, the gene product of the reporter genes will be expressed if the sample contains the mycobacteria of interest, thereby diagnosing mycobacterial infection.
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公开(公告)号：US06225066B1
公开(公告)日：2001-05-01
申请号：US09426436
申请日：1999-10-25
IPC分类号： C12Q168
CPC分类号： C07K14/005 , C07K14/35 , C12N7/00 , C12N15/74 , C12N2795/10322 , C12Q1/18 , C12Q1/6897 , G01N33/5695
摘要： This invention relates to mycobacterial species-specific reporter mycobacteriophages (reporter mycobacteriophages), methods of producing said reporter mycobacteriophages and the use of said reporter mycobacteriophages for the rapid diagnosis of mycobacterial infection and the assessment of drug susceptibilities of mycobacterial strains in clinical samples. In particular, this invention is directed to the production and use of luciferase reporter mycobacteriophages to diagnose tuberculosis. The mycobacterial species-specific reporter mycobacteriophages comprise mycobacterial species-specific mycobacteriophages which contain reporter genes and transcriptional promoters therein. When the reporter mycobacteriophages are incubated with clinical samples which may contain the mycobacteria of interest, the gene product of the reporter genes will be expressed if the sample contains the mycobacteria of interest, thereby diagnosing mycobacterial infection.
摘要翻译： 本发明涉及分枝杆菌物种特异性报道分枝杆菌噬菌体（报道分枝杆菌噬菌体），产生所述报道分枝杆菌噬菌体的方法和所述报道分枝杆菌噬菌体用于快速诊断分枝杆菌感染和评估临床样品中分枝杆菌菌株药物敏感性的用途。 特别地，本发明涉及荧光素酶报道分枝杆菌噬菌体的生产和使用以诊断结核病。 分枝杆菌种特异性报道分枝杆菌噬菌体包含含有报道基因和转录促进剂的分枝杆菌种特异性分枝杆菌噬菌体。 当报道分枝杆菌噬菌体与可能含有感兴趣分枝杆菌的临床样品一起孵育时，如果样品含有感兴趣的分枝杆菌，报告基因的基因产物将被表达，从而诊断分枝杆菌感染。
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公开(公告)号：US5750384A
公开(公告)日：1998-05-12
申请号：US247901
申请日：1994-05-23
CPC分类号： C07K14/005 , C07K14/35 , C12N15/74 , C12N7/00 , C12Q1/6897 , C12N2795/10311 , C12N2795/10322 , C12N2795/10343
摘要： This invention is directed to L5 shuttle phasmids capable of delivering foreign DNA into mycobacteria and to methods of producing L5 shuttle phasmids. In addition, this invention is directed to a method of generating mycobacterial mutations and to a method of producing mycobacterial vaccines.
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公开(公告)号：US5994137A
公开(公告)日：1999-11-30
申请号：US075904
申请日：1998-05-11
CPC分类号： C07K14/005 , C07K14/35 , C12N15/74 , C12N7/00 , C12Q1/6897 , C12N2795/10311 , C12N2795/10322 , C12N2795/10343
摘要： This invention is directed to L5 shuttle phasmids capable of delivering foreign DNA into mycobacteria and to methods of producing L5 shuttle phasmids. In addition, this invention is directed to a method of generating mycobacterial mutations and to a method of producing mycobacterial vaccines.
摘要翻译： 本发明涉及能够将外来DNA转运到分枝杆菌中的L5穿梭质粒以及产生L5穿梭质粒的方法。 此外，本发明涉及产生分枝杆菌突变的方法和生产分枝杆菌疫苗的方法。
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公开(公告)号：US5972700A
公开(公告)日：1999-10-26
申请号：US938059
申请日：1997-09-26
CPC分类号： C12N15/74
摘要： The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the TM4 mycobacteriophage that introduces DNA of interest into mycobacteria, especially M. tuberculosis complex organisms and other slow growing mycobacteria. The present invention provides a recombinant mycobacterium which expresses a DNA of interest incorporated into its chromosome by a TM4 conditional shuttle phasmid containing the DNA of interest. The present invention further provides a mycobacterial auxotrophic mutant and a method of generating auxotrophic mutants.
摘要翻译： 本发明提供了一种通过将粘粒插入到TM4分枝杆菌噬菌体的非必需区域中构建的条件穿梭质粒，其将感兴趣的DNA引入分枝杆菌，特别是结核分枝杆菌复合生物体和其他缓慢生长的分枝杆菌。 本发明提供了一种重组分枝杆菌，其通过含有感兴趣的DNA的TM4条件穿梭载体表达感兴趣的DNA并入其染色体中。 本发明还提供了分枝杆菌营养缺陷突变体和产生营养缺陷突变体的方法。
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