发明授权
- 专利标题: Methods for regulating gene expression
- 专利标题(中): 调节基因表达的方法
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申请号: US485978申请日: 1995-06-07
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公开(公告)号: US5814618A公开(公告)日: 1998-09-29
- 发明人: Hermann Bujard , Manfred Gossen
- 申请人: Hermann Bujard , Manfred Gossen
- 申请人地址: DEX Ludwigshafen DEX Ludwigshafen
- 专利权人: BASF Aktiengesellschaft,Knoll Aktiengesellchaft
- 当前专利权人: BASF Aktiengesellschaft,Knoll Aktiengesellchaft
- 当前专利权人地址: DEX Ludwigshafen DEX Ludwigshafen
- 主分类号: A01K67/027
- IPC分类号: A01K67/027 ; A61K48/00 ; C07K14/00 ; C07K14/035 ; C07K14/195 ; C07K14/245 ; C07K19/00 ; C12N15/11 ; C12N15/63 ; C12N15/67 ; C12N15/85 ; C12N15/00
摘要:
Methods of regulating gene expression in subjects using tetracycline-responsive fusion proteins are disclosed. In one embodiment, the method involves introducing into a cell the subject a nucleic acid molecule encoding a fusion protein which inhibits transcription, the fusion protein comprising a first polypeptide which binds to a tet operator sequence, operatively linked to a heterologous second polypeptide which inhibits transcription in eukaryotic cells; and modulating the concentration of a tetracycline, or analogue thereof, in the subject. The first polypeptide can binds to a tet operator sequence in the absence, but not the presence, of tetracycline. Alternatively, the first polypeptide can binds to a tet operator sequence in the presence, but not the absence, of tetracycline. In another embodiment, the method of the invention involves obtaining a cell from a subject, introducing into the cell a first nucleic acid molecule which operatively links a gene to at least one tet operator sequence, introducing into the cell a second nucleic acid molecule encoding an inhibitory fusion protein of the invention to form a modified cell, administering the modified cell to the subject and modulating the concentration of a tetracycline, or analogue thereof, in the subject. The first and second nucleic acid molecules can be linked or can be separate molecules.