发明授权
- 专利标题： Heat resistant maltose phosphorylase, process for preparation thereof, bacteria used for preparation thereof, and methods for using the enzyme
- 专利标题（中）： 耐热麦芽糖磷酸酶，其制备方法，用于制备的细菌，以及使用该酶的方法
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申请号： US686647申请日： 1996-07-26
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公开(公告)号： US5827715A公开(公告)日： 1998-10-27
- 发明人: Keiko Ishii , Yasushi Inoue , Tetsuji Tomita
- 申请人： Keiko Ishii , Yasushi Inoue , Tetsuji Tomita
- 申请人地址： JPX Tokyo
- 专利权人： Showa Sangyo Co., Ltd.
- 当前专利权人： Showa Sangyo Co., Ltd.
- 当前专利权人地址： JPX Tokyo
- 优先权： JPX7-213005 19950731
- 主分类号： C12N9/12
- IPC分类号： C12N9/12 ; C12N1/20 ; C12N9/10 ; C12P19/12 ; C12P19/18 ; C12R1/07 ; C12N9/26 ; C12N9/34 ; C12P19/20
摘要：
This invention relates to heat resistant maltose phosphorylase having an activity of 80% or more of the one untreated after treated in a buffer of pH 6.0, at one temperature of 50.degree. to 60.degree. C. for 15 minutes, a process for preparation thereof, bacteria used for preparation thereof, and processes for preparation of .beta.-glucose-1-phosphoric and trehalose using the enzyme. By carrying out enzymatic reaction at high reaction temperatures using this enzyme, it is possible to prepare .beta.-glucose-1-phosphoric acid or trehalose industrially advantageously, with lowering of contamination with various germs and shortening of reaction time.
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