利索-全球专利检索

  1. 登录
  2. 注册
发明授权
US5856108A Biosynthesis of diazomelanin and diazoluminomelanin and methods thereof 失效
重氮木兰素和二叠氮木兰素的生物合成及其方法

Biosynthesis of diazomelanin and diazoluminomelanin and methods thereof
摘要：
There is provided a method for producing diazoluminomelanin (DALM) which comprises culturing in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, a microorganism containing nitrate reductase.Also provided is a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube and activating luminescence.Further, there is provided a method for producing diazomelanin (DM) which comprises culturing in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, a microorganism containing nitrate reductase.Yet further, there is provided a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube, adding luminol and activating luminescence.
摘要（中）：

提供了一种生产重氮酚蓝蛋白（DALM）的方法，该方法包括在合适的代谢条件下，在含硝酸盐，3-氨基-L-酪氨酸（3-AT）和鲁米诺的培养基中培养含有硝酸还原酶的微生物。 还提供了一种用于直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐，3-氨基-L-酪氨酸（3-AT）和 将鲁米诺转移到适当的代谢条件下，将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管并激活发光。 此外，提供了一种生产重氮木兰素（DM）的方法，其包括在适当的代谢条件下在含有硝酸盐和3-氨基-L-酪氨酸（3-AT）的培养基中培养含有硝酸还原酶的微生物。 此外，提供了一种直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐和3-氨基-L-酪氨酸（3- AT），将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管，加入鲁米诺并激活发光。

公开/授权文献
信息查询
Global Dossier Espacenet
0/0