Disclosed and claimed are toxins produced by novel Bacillus thuringiensis insolates designated B.t. PS92J, B.t. PS196S1, B.t. PS201L1; and B.t. PS201T6, which have dipteran and/or corn rootworm activity. Thus, the insolates, or mutants thereof, can be used to control such pests. Further, claimed are novel genes encoding these .delta.-emdotoxins, which can be expressed in other hosts, Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.