发明授权
- 专利标题： Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
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申请号： US695191申请日： 1996-08-07
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公开(公告)号： US6025155A公开(公告)日： 2000-02-15
- 发明人: Gyula Hadlaczky , Aladar A. Szalay
- 申请人： Gyula Hadlaczky , Aladar A. Szalay
- 申请人地址： CAX HUX
- 专利权人： Chromos Molecular Systems, Inc.,The Biological Research Center of the Hungarian Academy of Sciences
- 当前专利权人： Chromos Molecular Systems, Inc.,The Biological Research Center of the Hungarian Academy of Sciences
- 当前专利权人地址： CAX HUX
- 主分类号： A01H1/00
- IPC分类号： A01H1/00 ; A01H5/00 ; A01K67/027 ; A61K38/00 ; A61K48/00 ; C12N5/10 ; C12N15/09 ; C12N15/113 ; C12N15/62 ; C12N15/82 ; C12N15/85 ; C12N15/67 ; C12P21/00
摘要：
Methods for preparing cell lines that contain artificial chromosomes, methods for preparation of artificial chromosomes, methods for purification of artificial chromosomes, methods for targeted insertion of heterologous DNA into artificial chromosomes, and methods for delivery of the chromosomes to selected cells and tissues are provided. Also provided are cell lines for use in the methods, and cell lines and chromosomes produced by the methods. In particular, satellite artificial chromosomes that, except for inserted heterologous DNA, are substantially composed of heterochromatin are provided. Methods for use of the artificial chromosomes, including for gene therapy, production of gene products and production of transgenic plants and animals are also provided.
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