发明授权
US6060246A Reagent and method for isolation and detection of selected nucleic acid
sequences
失效
用于分离和检测所选择的核酸序列的试剂和方法
- 专利标题: Reagent and method for isolation and detection of selected nucleic acid sequences
- 专利标题(中): 用于分离和检测所选择的核酸序列的试剂和方法
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申请号: US969813申请日: 1997-11-13
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公开(公告)号: US6060246A公开(公告)日: 2000-05-09
- 发明人: James E. Summerton , Dwight D. Weller , John M. Wages, Jr.
- 申请人: James E. Summerton , Dwight D. Weller , John M. Wages, Jr.
- 申请人地址: OR Corvallis
- 专利权人: AVI BioPharma, Inc.
- 当前专利权人: AVI BioPharma, Inc.
- 当前专利权人地址: OR Corvallis
- 主分类号: C12N15/10
- IPC分类号: C12N15/10 ; C12Q1/68 ; C07H21/02 ; C07H21/04 ; C12N15/00
摘要:
The invention relates to compositions and methods for rapidly detecting or isolating a target nucleic acid sequence in a polynucleotide-containing sample. The sample is exposed to a rapid pairing reagent, which contains a rapid capture component, effective to rapidly and non-selectively bind polynucleotides, and a target specific probe, effective to selectively bind the target nucleic acid sequence. Selectively disrupting the binding between the capture component and polynucleotides leaves only target sequence bound to the rapid pairing reagent.
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