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    Reagent and method for isolation and detection of selected nucleic acid sequences
    1.
    发明授权
    Reagent and method for isolation and detection of selected nucleic acid sequences 失效
    用于分离和检测所选择的核酸序列的试剂和方法

    公开(公告)号：US6060246A

    公开(公告)日：2000-05-09

    申请号：US969813

    申请日：1997-11-13

    申请人： James E. Summerton ,  Dwight D. Weller ,  John M. Wages, Jr.

    发明人： James E. Summerton ,  Dwight D. Weller ,  John M. Wages, Jr.

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C12N15/1006

    摘要： The invention relates to compositions and methods for rapidly detecting or isolating a target nucleic acid sequence in a polynucleotide-containing sample. The sample is exposed to a rapid pairing reagent, which contains a rapid capture component, effective to rapidly and non-selectively bind polynucleotides, and a target specific probe, effective to selectively bind the target nucleic acid sequence. Selectively disrupting the binding between the capture component and polynucleotides leaves only target sequence bound to the rapid pairing reagent.

    摘要翻译： 本发明涉及用于在含多核苷酸的样品中快速检测或分离靶核酸序列的组合物和方法。 将样品暴露于快速配对试剂，其包含有效快速和非选择性结合多核苷酸的快速捕获组分和有效选择性结合靶核酸序列的靶特异性探针。 选择性地破坏捕获组分和多核苷酸之间的结合仅留下与快速配对试剂结合的靶序列。

    Polymer composition for delivering substances in living organisms
    2.
    发明授权
    Polymer composition for delivering substances in living organisms 失效
    用于在活生物体中输送物质的聚合物组合物

    公开(公告)号：US6030941A

    公开(公告)日：2000-02-29

    申请号：US848844

    申请日：1997-04-30

    申请人： James E. Summerton ,  Dwight D. Weller

    发明人： James E. Summerton ,  Dwight D. Weller

    IPC分类号： A61K47/48 ,  A01N37/18

    CPC分类号： A61K47/48092 ,  A61K47/48315

    摘要： Polymeric compositions effective for delivering compounds in living organisms are described. The compositions include polypeptides which exhibit solubility in both hydrophilic and lipophilic environments by undergoing a reversible pH-dependent transition from a low-pH, lipophilic form to a high-pH, hydrophilic form.

    摘要翻译： 描述了有效递送生物体中化合物的聚合物组合物。 组合物包括通过经历从低pH，亲脂性形式到高pH亲水形式的可逆pH依赖性转变而在亲水和亲油环境中表现出溶解性的多肽。

    Sequence-specific binding polymers for duplex nucleic acids
    3.
    发明授权
    Sequence-specific binding polymers for duplex nucleic acids 失效
    用于双链体核酸的序列特异性结合聚合物

    公开(公告)号：US5405938A

    公开(公告)日：1995-04-11

    申请号：US979158

    申请日：1992-11-23

    申请人： James E. Summerton ,  Dwight D. Weller

    发明人： James E. Summerton ,  Dwight D. Weller

    IPC分类号： C07H21/04 ,  A61K31/70 ,  A61K31/785 ,  A61K47/48 ,  A61P31/12 ,  A61P35/00 ,  C07H21/00 ,  C08G73/00 ,  C12Q1/68 ,  C08G59/00 ,  C08G65/00

    CPC分类号： C07H21/00 ,  A61K47/48023 ,  A61K47/48061 ,  A61K47/48084 ,  A61K47/48192 ,  C12Q1/6813 ,  C12Q1/6839 ,  B01J2219/0072

    摘要： The present invention describes a polymer composition effective to bind, in a sequence-specific manner to a target sequence of a duplex polynucleotide, at least two different-oriented Watson/Crick base-pairs at selected positions in the target sequence. The composition includes an uncharged backbone with 5- or 6-membered cyclic backbone structures and selected bases attached to the backbone structures effective to hydrogen bond specifically with different oriented base-pairs in the target sequence. Also disclosed are subunits useful for the construction of the polymer composition. The present invention further includes methods for (i) coupling a first free or polymer-terminal subunit, and (ii) isolating, from a liquid sample, a target duplex nucleic acid fragment having a selected sequence of base-pairs.

    摘要翻译： 本发明描述了一种聚合物组合物，其以序列特异性方式结合双链多核苷酸的靶序列，在目标序列的选定位置处至少两个不同取向的沃森/克里克碱基对。 该组合物包括具有5-或6-元环状主链结构的不带电荷的主链，并且连接于有效与靶序列中不同取向碱基对氢键特异性结合的主链结构的选定碱基。 还公开了可用于构建聚合物组合物的亚基。 本发明还包括以下方法：（i）将第一游离或聚合物末端亚基偶联，和（ii）从液体样品中分离具有选定的碱基对序列的靶双链体核酸片段。

    Polynucleotide reagent containing chiral subunits and methods of use
    5.
    发明授权
    Polynucleotide reagent containing chiral subunits and methods of use 失效
    含有手性亚单位的多核苷酸试剂及其使用方法

    公开(公告)号：US5521063A

    公开(公告)日：1996-05-28

    申请号：US15211

    申请日：1993-02-09

    申请人： James E. Summerton ,  Dwight D. Weller

    发明人： James E. Summerton ,  Dwight D. Weller

    IPC分类号： A61K47/48 ,  C07H21/00 ,  C07K14/00 ,  C08G79/02 ,  C12Q1/68 ,  C07D413/00 ,  C08G59/00

    CPC分类号： C07H21/00 ,  A61K47/48061 ,  A61K47/48084 ,  A61K47/48215 ,  C07K14/003 ,  C08G79/02 ,  C12Q1/6813 ,  C12Q1/682 ,  B01J2219/0072

    摘要： The present invention describes an assay system wherein target polynucleotide molecules are captured on a support by base-specific binding to support-bound polymers, which are themselves substantially uncharged, and the target polynucleotides can be detected on the basis of their backbone charge. The assay system may also include polycationic reporter molecules which are designed to bind to the fully charged analyte backbone, but not the uncharged (or substantially uncharged) polymer backbone. In one embodiment, the reporter molecules are composed of a polycationic moiety or tail designed to bind electrostatically to a fully charged polynucleotide, under conditions where the reporter does not bind to the less charged or uncharged binding polymer carried on the diagnostic reagent.

    摘要翻译： 本发明描述了一种测定系统，其中目标多核苷酸分子通过碱基特异性结合支持结合聚合物捕获在载体上，载体结合的聚合物本身基本上不带电荷，并且可以基于其主链电荷检测靶多核苷酸。 测定系统还可以包括聚阳离子报道分子，其被设计为结合完全带电的分析物主链，而不是不带电荷（或基本上不带电荷的）聚合物主链。 在一个实施方案中，报道分子由设计为静电结合到完全带电荷的多核苷酸的聚阳离子部分或尾部组成，其中报道分子不与携带在诊断试剂上的较少带电荷或不带电的结合聚合物结合的条件下。

    Alpha-morpholino ribonucleoside derivatives and polymers thereof
    6.
    发明授权
    Alpha-morpholino ribonucleoside derivatives and polymers thereof 失效
    阿尔法 - 吗啉基赖氨酸衍生物及其聚合物

    公开(公告)号：US5235033A

    公开(公告)日：1993-08-10

    申请号：US454056

    申请日：1989-12-20

    申请人： James E. Summerton ,  Dwight D. Weller ,  Eugene P. Stirchak

    发明人： James E. Summerton ,  Dwight D. Weller ,  Eugene P. Stirchak

    IPC分类号： A61K47/48 ,  C07H21/00 ,  C07K14/00 ,  C12Q1/68

    CPC分类号： C07H21/00 ,  A61K47/48061 ,  A61K47/48192 ,  C07K14/003 ,  C12Q1/6813 ,  C12Q1/682 ,  B01J2219/0072

    摘要： Alpha-morpholino subunits and polymer compositions composed of alpha-morpholino subunits are disclosed. These subunits can be linked together by uncharged linkages, one to three atoms in length, joining the morpholino nitrogen of one subunit to the 5' exocyclic carbon of an adjacent subunit. Each subunit contains a purine or pyrimidine base-pairing moiety effective to bind by hydrogen bonding to a specific base or base-pair in a target polynucleotide. The polymers of the present invention can be used in place of standard RNA or DNA oligomers for a number of standard laboratory procedures, for example, as probes in solid-phase systems and nucleic acid hybridization analyses. Further, the polymers of the present invention have potential use as therapeutic agents in anti-sense-type technologies.

    摘要翻译： 公开了α-吗啉代亚基和由α-吗啉代亚基组成的聚合物组合物。 这些亚基可以通过不带电荷的键连接在一起，长度为1-3个原子，将一个亚基的吗啉代氮连接到相邻亚基的5'环外碳上。 每个亚基含有通过氢键与靶多核苷酸中的特定碱基或碱基对有效结合的嘌呤或嘧啶碱基配对部分。 本发明的聚合物可用于代替多种标准实验室程序的标准RNA或DNA寡聚体，例如作为固相体系和核酸杂交分析中的探针。 此外，本发明的聚合物在反义型技术中具有潜在的用途作为治疗剂。

    Alpha-morpholino ribonucleoside derivatives and polymers thereof
    9.
    发明授权
    Alpha-morpholino ribonucleoside derivatives and polymers thereof 失效
    α-吗啉代核糖核苷衍生物及其聚合物

    公开(公告)号：US5378841A

    公开(公告)日：1995-01-03

    申请号：US74120

    申请日：1993-06-08

    申请人： James E. Summerton ,  Dwight D. Weller ,  Eugene P. Stirchak

    发明人： James E. Summerton ,  Dwight D. Weller ,  Eugene P. Stirchak

    IPC分类号： C07D417/14 ,  C07D473/00 ,  C07H21/00 ,  C12Q1/68 ,  C07D413/04 ,  C07D473/02 ,  C07D473/26

    CPC分类号： C07D417/14 ,  C07D473/00 ,  C07H21/00 ,  C12Q1/68

    摘要： Alpha-morpholino subunits and polymer compositions composed of alpha-morpholino subunits are disclosed. These subunits can be linked together by uncharged linkages, one to three atoms in length, joining the morpholino nitrogen of one subunit to the 5' exocyclic carbon of an adjacent subunit. Each subunit contains a purine or pyrimidine base-pairing moiety effective to bind by hydrogen bonding to a specific base or base-pair in a target polynucleotide. The polymers of the instant invention can be used in place of standard RNA or DNA oligomers. For example, the target-specific polymers of the invention can be used in a variety of diagnostic assays for detection of RNA or DNA having a given target sequence. Further, the polymers of the invention also have potential use as therapeutic agents.

    摘要翻译： 公开了α-吗啉代亚基和由α-吗啉代亚基组成的聚合物组合物。 这些亚基可以通过不带电荷的键连接在一起，长度为1-3个原子，将一个亚基的吗啉代氮连接到相邻亚基的5'环外碳上。 每个亚基含有通过氢键与靶多核苷酸中的特定碱基或碱基对有效结合的嘌呤或嘧啶碱基配对部分。 本发明的聚合物可以用于代替标准的RNA或DNA寡聚体。 例如，本发明的靶标特异性聚合物可用于各种用于检测具有给定靶序列的RNA或DNA的诊断测定。 此外，本发明的聚合物也具有潜在的用途作为治疗剂。