发明授权
- 专利标题： Sequence modification of oligonucleotide primers to manipulate non-templated nucleotide addition
- 专利标题（中）： 寡核苷酸引物的序列修饰以操纵非模板核苷酸添加
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申请号： US51620申请日： 1998-04-14
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公开(公告)号： US6110710A公开(公告)日： 2000-08-29
- 发明人: Jeffrey R. Smith , John D. Carpten , Michael J. Brownstein
- 申请人： Jeffrey R. Smith , John D. Carpten , Michael J. Brownstein
- 申请人地址： DC Washington
- 专利权人： The United States of America as represented by the Secretary of the Department of Health and Human Services
- 当前专利权人： The United States of America as represented by the Secretary of the Department of Health and Human Services
- 当前专利权人地址： DC Washington
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C12P19/34 ; C12Q1/68
摘要：
The present invention is directed to methods for resisting or promoting template independent nucleotide addition to the 3' terminus of a DNA duplex. The process comprises amplifying a target nucleic acid using primers which comprise a 5' terminal sequence which resists or promotes non-templated nucleotide addition to the 3' terminus of the complementary nucleic acid strand. The invention is also directed to a kit for cloning 3' nucleotidylated duplex DNA.
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