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    Sequence modification of oligonucleotide primers to manipulate non-templated nucleotide addition
    3.
    发明授权
    Sequence modification of oligonucleotide primers to manipulate non-templated nucleotide addition 失效
    寡核苷酸引物的序列修饰以操纵非模板核苷酸添加

    公开(公告)号：US6110710A

    公开(公告)日：2000-08-29

    申请号：US51620

    申请日：1998-04-14

    申请人： Jeffrey R. Smith ,  John D. Carpten ,  Michael J. Brownstein

    发明人： Jeffrey R. Smith ,  John D. Carpten ,  Michael J. Brownstein

    IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12P19/34 ,  C12N15/10 ,  C12Q1/686

    摘要： The present invention is directed to methods for resisting or promoting template independent nucleotide addition to the 3' terminus of a DNA duplex. The process comprises amplifying a target nucleic acid using primers which comprise a 5' terminal sequence which resists or promotes non-templated nucleotide addition to the 3' terminus of the complementary nucleic acid strand. The invention is also directed to a kit for cloning 3' nucleotidylated duplex DNA.

    摘要翻译： PCT No.PCT / US96 / 16544 Sec。 371日期1998年6月15日第 102（e）1998年6月15日PCT PCT 1996年10月15日PCT公布。 公开号WO97 / 16566 日期1997年5月9日本发明涉及抵抗或促进与DNA双链体的3'端的模板无关核苷酸添加的方法。 该方法包括使用包含5'末端序列的引物扩增靶核酸，所述末端序列抵抗或促进互补核酸链的3'末端的非模板核苷酸添加。 本发明还涉及用于克隆3'核苷酸化双链体DNA的试剂盒。

    Array-type multiple cell injector
    4.
    发明授权
    Array-type multiple cell injector 失效
    阵列式多单元注射器

    公开(公告)号：US5262128A

    公开(公告)日：1993-11-16

    申请号：US425254

    申请日：1989-10-23

    申请人： Stephen B. Leighton ,  Michael J. Brownstein

    发明人： Stephen B. Leighton ,  Michael J. Brownstein

    IPC分类号： B01L3/00 ,  B01L3/02 ,  C12M3/00 ,  C12N15/87 ,  G01N35/00 ,  C12M1/26

    CPC分类号： C12N15/87 ,  B01L3/0244 ,  B01L3/0248 ,  B01L3/5085 ,  C12M23/12 ,  C12M23/50 ,  C12M29/00 ,  C12M35/00 ,  B01L2300/0829 ,  B01L2400/0487 ,  G01N2035/00237 ,  Y10S436/809 ,  Y10T436/2575

    摘要： An injection device injects small amounts of injectate into each of a plurality of cells. A first plate, which may be made from a silicon chip, includes a large number of evenly spaced cell wells, in a regular array. Each well includes a through hole in the chip. A top plate, also formed from a silicon chip, includes a plurality of injection needles, arranged in a regular array corresponding to the position of the cell wells. A suspension of cells is washed over the first plate and individual cells are retained by the individual wells. A vacuum may be applied to the cell wells through a manifold beneath the first plate, thus to retain the cells in place. Excess cells and suspension are removed by washing the plate. The second plate, including the injection needles, is positioned on the first plate so that the needles pierce the plurality of cells retained in the wells, thus simultaneously introducing the injectate into a large number of individual cells.

    摘要翻译： 注射装置将少量的注射液注入多个细胞的每一个中。 可以由硅芯片制成的第一板包括大量均匀间隔的电池槽，以规则排列。 每个井都包括芯片中的通孔。 也由硅芯片形成的顶板包括多个注射针，其排列成与细胞孔的位置对应的规则阵列。 细胞悬浮液在第一个平板上洗涤，各个细胞由各个孔保留。 可以通过第一板下面的歧管向细胞阱施加真空，从而将细胞保持在适当位置。 通过洗涤板除去过量的电池和悬浮液。 包括注射针在内的第二板定位在第一板上，使得针刺入保留在孔中的多个细胞，从而同时将注射器引入大量的单个细胞中。

    CDNA clone of a rat serotonin transporter and protein encoded thereby
    7.
    发明授权
    CDNA clone of a rat serotonin transporter and protein encoded thereby 失效
    大鼠血清素转运蛋白的CDNA克隆和由此编码的蛋白质

    公开(公告)号：US5552308A

    公开(公告)日：1996-09-03

    申请号：US782298

    申请日：1991-10-24

    申请人： Beth J. Hoffman ,  Eva Mezey ,  Michael J. Brownstein

    发明人： Beth J. Hoffman ,  Eva Mezey ,  Michael J. Brownstein

    IPC分类号： A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C12N15/12

    CPC分类号： C07K14/70571 ,  C07K14/47 ,  A61K38/00

    摘要： The invention described in this disclosure relates to a cloned cDNA encoding the serotonin transporter protein (5HTT) usually found in cells of part of the central nervous system, gut, adrenal gland and in platelets. The invention is further directed to the purified serotonin transporter protein and its use and immunogen for the production of anti-5HTT antibodies. The disclosure also discussses methods for use of the cDNA for diagnostic and treatment applications, and methods for use of permanent cell lines transformed with the serotonin transporter cDNA for pharmaceutical screening. The use of anti-5HTT antibodies as a diagnostic tool is also addressed.

    摘要翻译： 本公开中描述的本发明涉及编码通常存在于部分中枢神经系统，肠，肾上腺和血小板的细胞中的5-羟色胺转运蛋白（5HTT）的克隆cDNA。 本发明还涉及纯化的血清素转运蛋白及其用于产生抗5HTT抗体的用途和免疫原。 本公开还讨论了用于诊断和治疗应用的cDNA的方法，以及使用用血清素转运蛋白cDNA转化用于药物筛选的永久性细胞系的方法。 还讨论了使用抗5HTT抗体作为诊断工具。