发明授权
US6143952A Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific
polyhydroxyalkanoate polymerase
失效
编码双特异性聚羟基链烷酸酯聚合酶的改良油菜素phaC1核酸
- 专利标题： Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase
- 专利标题（中）： 编码双特异性聚羟基链烷酸酯聚合酶的改良油菜素phaC1核酸
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申请号： US52689申请日： 1998-03-31
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公开(公告)号： US6143952A公开(公告)日： 2000-11-07
- 发明人: Friedrich Srienc , John K. Jackson , David A. Somers
- 申请人： Friedrich Srienc , John K. Jackson , David A. Somers
- 申请人地址： MN Minneapolis
- 专利权人： Regents of the University of Minnesota
- 当前专利权人： Regents of the University of Minnesota
- 当前专利权人地址： MN Minneapolis
- 主分类号： C12N9/10
- IPC分类号： C12N9/10 ; C12N15/31 ; C12P7/62 ; A01H5/00 ; C12N1/21 ; C12N15/52 ; C12N15/70 ; C12N15/81 ; C12N15/82
摘要：
A genetically engineered Pseudomonas oleovorans phaC1 polyhydroxyalkanoate (PHA) polymerase having tailored substrate specificity is provided. The modified PHA polymerase is preferably a "bispecific" PHA polymerase capable of copolymerizing a short chain length monomer and a medium chain length monomer is provided. Methods for making the modified PHA polymerase and for making nucleic acids encoding the modified PHA polymerase are also disclosed, as are methods of producing PHA using the modified PHA polymerase. The invention further includes methods to assay for altered substrate specificity.
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