发明授权
US06177270B1 Reaction-based selection for expression of and concentration of catalytic moieties
失效
用于催化部分的表达和浓缩的基于反应的选择
- 专利标题: Reaction-based selection for expression of and concentration of catalytic moieties
- 专利标题(中): 用于催化部分的表达和浓缩的基于反应的选择
-
申请号: US09235353申请日: 1999-01-21
-
公开(公告)号: US06177270B1公开(公告)日: 2001-01-23
- 发明人: Mark T. Martin , Rodger G. Smith , Michael J. Darsley , David M. Simpson , Gary F. Blackburn
- 申请人: Mark T. Martin , Rodger G. Smith , Michael J. Darsley , David M. Simpson , Gary F. Blackburn
- 主分类号: C12N700
- IPC分类号: C12N700
摘要:
Disclosed and claimed are methods for selecting a recombinant virus, phage or cell expressing a catalytic antibody or catalytic portion thereof, or for selecting catalytic activity by a moiety. The method employs reaction-based selection for catalytic activity. The method can also be used to concentrate (increase the proportion of catalytic to non-catalytic moieties) a sample containing a catalytic moiety or viruses, phages or cells expressing a catalytic moiety. The selection or concentrating can be by employing a mechanism-based inhibitor, catalysis-accelerated movement, surface binding, changes in enthalpic component of binding as a function of temperature, or changes in binding by competition, or combinations thereof. The invention also comprehends a method for producing a recombinant virus or a cell-line expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof; and, this method can include infecting a suitable host with viruses which are screened for the expression. In addition, recombinant viruses and cell-lines so expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof are also disclosed and claimed.