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7 条记录 (耗时 0.045 秒)

    Reaction-based selection for expression of and concentration of catalytic moieties
    1.
    发明授权
    Reaction-based selection for expression of and concentration of catalytic moieties 失效
    用于催化部分的表达和浓缩的基于反应的选择

    公开(公告)号：US06177270B1

    公开(公告)日：2001-01-23

    申请号：US09235353

    申请日：1999-01-21

    申请人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

    发明人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

    IPC分类号： C12N700

    CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12Q1/02 ,  C12Q1/6811 ,  C12Q1/70 ,  G01N33/56983

    摘要： Disclosed and claimed are methods for selecting a recombinant virus, phage or cell expressing a catalytic antibody or catalytic portion thereof, or for selecting catalytic activity by a moiety. The method employs reaction-based selection for catalytic activity. The method can also be used to concentrate (increase the proportion of catalytic to non-catalytic moieties) a sample containing a catalytic moiety or viruses, phages or cells expressing a catalytic moiety. The selection or concentrating can be by employing a mechanism-based inhibitor, catalysis-accelerated movement, surface binding, changes in enthalpic component of binding as a function of temperature, or changes in binding by competition, or combinations thereof. The invention also comprehends a method for producing a recombinant virus or a cell-line expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof; and, this method can include infecting a suitable host with viruses which are screened for the expression. In addition, recombinant viruses and cell-lines so expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof are also disclosed and claimed.

    摘要翻译： 公开并要求的是选择重组病毒，表达催化抗体或其催化部分的噬菌体或细胞，或用于通过部分选择催化活性的方法。 该方法采用基于反应的催化活性选择。 该方法还可用于浓缩（增加非催化部分的催化剂比例）含有催化部分或表达催化部分的病毒，噬菌体或细胞的样品。 选择或浓缩可以通过采用基于机理的抑制剂，催化加速运动，表面结合，作为温度的函数的结合焓分量的变化，或通过竞争的结合变化，或其组合。 本发明还包括生产重组病毒或表达催化部分如催化抗体或其催化部分的细胞系的方法; 并且该方法可以包括用筛选表达的病毒感染合适的宿主。 此外，还公开和要求保护了重组病毒和表达催化部分如催化抗体或其催化部分的细胞系。

    Reaction-based selection for expression of and concentration of catalytic moieties
    2.
    发明授权
    Reaction-based selection for expression of and concentration of catalytic moieties 失效
    用于催化部分的表达和浓缩的基于反应的选择

    公开(公告)号：US5631137A

    公开(公告)日：1997-05-20

    申请号：US377495

    申请日：1995-01-24

    申请人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

    发明人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

    IPC分类号： G01N33/53 ,  B01J20/281 ,  C12N5/10 ,  C12N7/02 ,  C12N15/10 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/70 ,  C40B40/02 ,  G01N30/88 ,  G01N33/569 ,  C12Q1/25 ,  C12N9/00

    CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12Q1/02 ,  C12Q1/6811 ,  C12Q1/70 ,  G01N33/56983

    摘要： Disclosed and claimed are methods for selecting a recombinant virus, phage or cell expressing a catalytic antibody or catalytic portion thereof, or for selecting catalytic activity by a moiety. The method employs reaction-based selection for catalytic activity. The method can also be used to concentrate (increase the proportion of catalytic to non-catalytic moieties) a sample containing a catalytic moiety or viruses, phages or cells expressing a catalytic moiety. The selection or concentrating can be by employing a mechanism-based inhibitor, catalysis-accelerated movement, surface binding, changes in enthalpic component of binding as a function of temperature, or changes in binding by competition, or combinations thereof. The invention also comprehends a method for producing a recombinant virus or a cell-line expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof; and, this method can include infecting a suitable host with viruses which are screened for the expression. In addition, recombinant viruses and cell-lines so expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof are also disclosed and claimed.

    摘要翻译： 公开并要求的是选择重组病毒，表达催化抗体或其催化部分的噬菌体或细胞，或用于通过部分选择催化活性的方法。 该方法采用基于反应的催化活性选择。 该方法还可用于浓缩（增加非催化部分的催化剂比例）含有催化部分或表达催化部分的病毒，噬菌体或细胞的样品。 选择或浓缩可以通过采用基于机理的抑制剂，催化加速运动，表面结合，作为温度的函数的结合焓分量的变化，或通过竞争的结合变化，或其组合。 本发明还包括生产重组病毒或表达催化部分如催化抗体或其催化部分的细胞系的方法; 并且该方法可以包括用筛选表达的病毒感染合适的宿主。 此外，还公开和要求保护了重组病毒和表达催化部分如催化抗体或其催化部分的细胞系。

    Reaction-based selection for expression of and concentration of catalytic moieties
    3.
    发明授权
    Reaction-based selection for expression of and concentration of catalytic moieties 失效
    用于催化部分的表达和浓缩的基于反应的选择

    公开(公告)号：US6121007A

    公开(公告)日：2000-09-19

    申请号：US747654

    申请日：1996-11-13

    申请人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

    发明人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

    IPC分类号： G01N33/53 ,  B01J20/281 ,  C12N5/10 ,  C12N7/02 ,  C12N15/10 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/70 ,  C40B40/02 ,  G01N30/88 ,  G01N33/569 ,  C12Q1/25

    CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12Q1/02 ,  C12Q1/6811 ,  C12Q1/70 ,  G01N33/56983

    摘要： Disclosed and claimed are methods for selecting a recombinant virus, phage or cell expressing a catalytic antibody or catalytic portion thereof, or for selecting catalytic activity by a moiety. The method employs reaction-based selection for catalytic activity. The method can also be used to concentrate (increase the proportion of catalytic to non-catalytic moieties) a sample containing a catalytic moiety or viruses, phages or cells expressing a catalytic moiety. The selection or concentrating can be by employing a mechanism-based inhibitor, catalysis-accelerated movement, surface binding, changes in enthalpic component of binding as a function of temperature, or changes in binding by competition, or combinations thereof. The invention also comprehends a method for producing a recombinant virus or a cell-line expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof; and, this method can include infecting a suitable host with viruses which are screened for the expression. In addition, recombinant viruses and cell-lines so expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof are also disclosed and claimed.

    摘要翻译： 公开并要求的是选择重组病毒，表达催化抗体或其催化部分的噬菌体或细胞，或用于通过部分选择催化活性的方法。 该方法采用基于反应的催化活性选择。 该方法还可用于浓缩（增加非催化部分的催化剂比例）含有催化部分或表达催化部分的病毒，噬菌体或细胞的样品。 选择或浓缩可以通过采用基于机理的抑制剂，催化加速运动，表面结合，作为温度的函数的结合焓分量的变化，或通过竞争的结合变化，或其组合。 本发明还包括生产重组病毒或表达催化部分如催化抗体或其催化部分的细胞系的方法; 并且该方法可以包括用筛选表达的病毒感染合适的宿主。 此外，还公开和要求保护了重组病毒和表达催化部分如催化抗体或其催化部分的细胞系。

    Lipid-A analogs: monosaccharide and dissaccharide compounds for inhibiting binding of lipid A receptors to lipid A receptors
    6.
    发明授权
    Lipid-A analogs: monosaccharide and dissaccharide compounds for inhibiting binding of lipid A receptors to lipid A receptors 失效
    脂质-A类似物：用于抑制脂质A受体与脂质A受体结合的单糖和非糖化合物

    公开(公告)号：US5593969A

    公开(公告)日：1997-01-14

    申请号：US123590

    申请日：1993-09-17

    申请人： Balreddy Kamireddy ,  Michael J. Darsley ,  David M. Simpson ,  Richard J. Massey

    发明人： Balreddy Kamireddy ,  Michael J. Darsley ,  David M. Simpson ,  Richard J. Massey

    IPC分类号： A61K38/00 ,  C07H13/06 ,  C07H15/04 ,  C07K16/12 ,  A61K31/70 ,  C07H3/02 ,  C07H3/04

    CPC分类号： C07H13/06 ,  C07H15/04 ,  C07K16/1203 ,  A61K38/00

    摘要： A compound of the formula: ##STR1## wherein: each of R.sub.1, R.sub.1 ', R.sub.2 and R.sub.2 ' independent of each other is a substituted or unsubstituted, branched or linear C.sub.1-12 alkyl, alkene or alkyne group, R.sub.3 is OH, OCH.sub.3, CH.sub.2 COOH or ##STR2## wherein each of R.sub.2" and R.sub.2 '41 independent of each other is a substituted or unsubstituted, branched or linear C.sub.1-12 alkyl, alkene or alkyne group and:A=NH.sub.2, X=P(OH), Y=Z=C, B=OCH.sub.3, orA=OH, X=P(OH), X=Z=C, B (if present)=OCH.sub.3, orA=OCO(CH.sub.2).sub.n NH.sub.2, X=P(OH), Y=Z=C, B=OCH.sub.3,wherein n=1-10, orA=OH, X=P(OH), Y=Z=C, B=O(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-10, orA=OH, X=P(OH), Y=Z=C, B=(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-10, orA=NH.sub.2, X=Z=C, Y=P(OH), B=OCH.sub.3, orA=OH, X=Z=C, Y=P(OH), B (if present)=OCH.sub.3, orA=OCO(CH.sub.2).sub.n NH.sub.2, X=Z=C, Y=P(OH), B=OCH.sub.3, wherein n=1-10, orA=OH, X=Z=C, Y=P(OH), B=O(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-10, orA=OH, X=Z=C, Y=P(OH), B=(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-11, orA=NH.sub.2, X=Y=C, Z=P(OH), B=OCH.sub.3, orA=OH, X=Y=C, Z=P(OH), B (if present)=OCH.sub.3, orA=OCO(CH.sub.2).sub.n NH.sub.2, X=Y=C, Z=P(OH), B=OCH.sub.3, wherein n=1-10, orA=OH, X=Y=C, Z=P(OH), B=O(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-10, orA=OH, X=Y=C, Z=P (OH), B=(CH.sub.2).sub.n CO.sub.2 H and n=1-11 is disclosed. The compounds may be use to inhibit binding of Lipid A to Lipid A receptors.

    摘要翻译： 下式的化合物：其中：R 1，R 1'，R 2和R 2'彼此独立地为取代或未取代的支链或直链C 1-12烷基，烯烃或炔基，R 3为 OH，OCH 3，CH 2 COOH或者其中每个R 2“和R 21'彼此独立地是取代或未取代的支链或直链C 1-12烷基，烯烃或炔基，并且：A = NH 2，X = P （OH），Y = Z = C，B = OCH 3或A = OH，X = P（OH），X = Z = C，B（如果存在）= OCH 3或A = OCO（CH 2）n NH 2，X = P（OH），Y = Z = C，B = OCH 3，其中n = 1-10，或A = OH，X = P（OH），Y = Z = C，B = O（CH 2）n CO 2 H，其中 n = 1-10，或A = OH，X = P（OH），Y = Z = C，B =（CH2）nCO2H，其中n = 1-10，或A = NH2，X = Z = C，Y = P（OH），B = OCH3或A = OH，X = Z = C，Y = P（OH），B（如果存在）= OCH 3或A = OCO（CH 2）n NH 2，X = Z = C ，Y = P（OH），B = OCH 3，其中n = 1-10，或A = OH，X = Z = C，Y = P（OH），B = O（CH 2）n CO 2 H，其中n = 10或A = OH，X = Z = C，Y = P（OH），B =（CH2）nCO2H，其中n = 1-11或A = NH2，X = Y = C，Z = P ），B = OCH 3或A = OH，X = Y = C，Z = P（OH），B（如果存在）= OCH 3或A = OCO（CH 2）n NH 2，X = Y = C，Z = P （OH），B = OCH 3， 其中n = 1-10，或A = OH，X = Y = C，Z = P（OH），B = O（CH 2）n CO 2 H，其中n = 1-10，或A = OH，X = Y = C ，Z = P（OH），B =（CH2）nCO2H和n = 1-11。 该化合物可用于抑制脂质A与脂质A受体的结合。