发明授权
US06211435B1 Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
有权
氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法
- 专利标题： Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
- 专利标题（中）： 氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法
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申请号： US09352168申请日： 1999-07-12
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公开(公告)号： US06211435B1公开(公告)日： 2001-04-03
- 发明人: Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Oswald R. Crasta , Otto Folkerts
- 申请人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Oswald R. Crasta , Otto Folkerts
- 主分类号： C12N504
- IPC分类号： C12N504
摘要：
The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.