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16 条记录 (耗时 0.029 秒)

    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    1.
    发明授权
    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 失效
    氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法

    公开(公告)号：US06943279B1

    公开(公告)日：2005-09-13

    申请号：US09658835

    申请日：2000-09-08

    申请人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Aragula Gururaj Rao ,  Oswald R. Crasta ,  Otto Folkerts

    发明人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Aragula Gururaj Rao ,  Oswald R. Crasta ,  Otto Folkerts

    IPC分类号： C12N9/06 ,  C12N15/09 ,  C12N15/31 ,  C12N15/82 ,  A10H1/00 ,  A10H5/10

    CPC分类号： C12N9/0022 ,  C12N15/8242 ,  C12N15/8282

    摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella airovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

    摘要翻译： 本发明提供了从Exophiala spinifera和Rhinocladiella airovirens分离的酶APAO的多核苷酸和相关多肽。 可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。 另外，本发明提供了重组表达盒，宿主细胞，转基因植物和转基因种子。 本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。 此外，本发明提供了在原核和真核系统中生产APAO酶的方法，用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。 还公开了降解植物，谷物，谷物加工，青贮饲料，粮食作物和动物饲料中真菌毒素的方法。

    Nitric oxide as an activator of the plant pathogen defense systems
    2.
    发明授权
    Nitric oxide as an activator of the plant pathogen defense systems 有权
    一氧化氮作为植物病原体防御系统的活化剂

    公开(公告)号：US06198020B1

    公开(公告)日：2001-03-06

    申请号：US09257541

    申请日：1999-02-24

    申请人： Benjamin A. Bowen ,  Jonathan P. Duvick ,  Carl R. Simmons

    发明人： Benjamin A. Bowen ,  Jonathan P. Duvick ,  Carl R. Simmons

    IPC分类号： A01H100

    CPC分类号： C12N9/0075 ,  C07K14/415 ,  C07K14/47 ,  C12N15/8243 ,  C12N15/8279

    摘要： Compositions and methods to aid in protecting the plant from invading pathogenic organisms are provided. The compositions of the invention comprise genes that influence the levels of nitric oxide in plant cells. Such genes include those encoding nitric oxide synthase (iNOS) as well as natural resistance-associated macrophage proteins (NRAMP) and NRAMP homologues. A pathogen inducible promoter or alternatively a constitutive, preferably a weak constitutive, promoter is used to control the desired level of disease control in the plant. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.

    摘要翻译： 提供了有助于保护植物免受入侵病原生物体的组合物和方法。 本发明的组合物包含影响植物细胞中一氧化氮水平的基因。 这些基因包括编码一氧化氮合酶（iNOS）以及天然抗性相关巨噬细胞蛋白（NRAMP）和NRAMP同源物的基因。 病原体诱导型启动子或选择性的组成型优选弱组成型启动子用于控制植物中所需的疾病控制水平。 还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。

    Amino polyol oxidase amine polynucleotides and related polypeptides and methods of use
    3.
    发明授权
    Amino polyol oxidase amine polynucleotides and related polypeptides and methods of use 失效
    氨基多胺胺氧化酶胺多核苷酸及相关多肽及其使用方法

    公开(公告)号：US06835569B2

    公开(公告)日：2004-12-28

    申请号：US09770564

    申请日：2001-01-26

    申请人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Oswald Crasta ,  Otto Folkerts

    发明人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Oswald Crasta ,  Otto Folkerts

    IPC分类号： C12N1509

    CPC分类号： C12N9/0022 ,  A23K10/14 ,  A23K10/18 ,  A23K30/18 ,  C07K2319/00 ,  C12N9/16 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282 ,  G01N2333/906

    摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems.

    摘要翻译： 本发明提供了从Exophiala spinifera分离的酶APAO的多核苷酸和相关多肽。 此外，编码APAO酶的多核苷酸可用于转化通常易受镰刀菌或其它产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 另外，本发明提供在转基因植物中表达APAO和伏马菌素酯酶。 以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。 此外，本发明提供了在原核和非植物真核系统中生产APAO酶的方法。

    Sclerotinia-inducible promoters and their uses
    4.
    发明授权
    Sclerotinia-inducible promoters and their uses 失效
    核盘菌诱导型启动子及其用途

    公开(公告)号：US06667427B1

    公开(公告)日：2003-12-23

    申请号：US09685292

    申请日：2000-10-10

    申请人： Zhangmeng Bao ,  Jonathan P. Duvick ,  Xu Hu ,  Guihua Lu

    发明人： Zhangmeng Bao ,  Jonathan P. Duvick ,  Xu Hu ,  Guihua Lu

    IPC分类号： C12N509

    CPC分类号： C07K14/415 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/8282

    摘要： Compositions and methods to aid in protecting plants from invading pathogenic organisms are provided. The compositions of the invention comprise anti-pathogenic nucleotide sequences, including their promoters, and polypeptides encoded by the anti-pathogenic nucleic acid sequences. The compositions find use in methods for reducing or eliminating damage to plants caused by plant pathogens. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.

    摘要翻译： 提供了有助于保护植物免受入侵病原生物体的组合物和方法。 本发明的组合物包含抗致病核苷酸序列，包括其启动子和由抗病原核酸序列编码的多肽。 该组合物用于减少或消除由植物病原体引起的植物损伤的方法。 还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。

    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    5.
    发明授权
    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权
    氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法

    公开(公告)号：US06211435B1

    公开(公告)日：2001-04-03

    申请号：US09352168

    申请日：1999-07-12

    申请人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Oswald R. Crasta ,  Otto Folkerts

    发明人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Oswald R. Crasta ,  Otto Folkerts

    IPC分类号： C12N504

    CPC分类号： C12N9/0022 ,  A23K10/14 ,  A23K10/18 ,  A23K30/18 ,  C07K2319/00 ,  C12N9/16 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282 ,  G01N2333/906

    摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

    摘要翻译： 本发明提供了从Exophiala spinifera分离的酶APAO的多核苷酸和相关多肽。 此外，编码APAO酶的多核苷酸可用于转化通常易受镰刀菌或其它产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 另外，本发明提供在转基因植物中表达APAO和伏马菌素酯酶。 以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。 此外，本发明提供了在原核和非植物真核系统中生产APAO酶的方法。 还公开了谷物，谷物加工，青贮饲料，粮食作物，动物饲料和瘤胃微生物的解毒方法。

    Rac-like genes and methods of use
    6.
    发明授权
    Rac-like genes and methods of use 有权
    类似Rac的基因和使用方法

    公开(公告)号：US06555732B1

    公开(公告)日：2003-04-29

    申请号：US09391741

    申请日：1999-09-08

    申请人： Jonathan P. Duvick ,  Yogesh Kumar Sharma

    发明人： Jonathan P. Duvick ,  Yogesh Kumar Sharma

    IPC分类号： C12N1529

    CPC分类号： C12N15/8242 ,  C12N15/8279

    摘要： The present invention provides methods and compositions relating to creating or enhancing disease resistance in plants. The invention provides isolated maize Rac nucleic acids and their encoded proteins that are involved in the altering the disease resistance pathway in plants, increasing transformation efficiency, inducing programmed cell death, and modulating the oxidative burst in a plant. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

    摘要翻译： 本发明提供了关于在植物中产生或增强抗病性的方法和组合物。 本发明提供分离的玉米Rac核酸及其编码的蛋白质，其参与改变植物中的抗病性途径，提高转化效率，诱导程序性细胞死亡和调节植物中的氧化突发。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。