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    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    4.
    发明授权
    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权
    氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法

    公开(公告)号：US06211435B1

    公开(公告)日：2001-04-03

    申请号：US09352168

    申请日：1999-07-12

    申请人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Oswald R. Crasta ,  Otto Folkerts

    发明人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Oswald R. Crasta ,  Otto Folkerts

    IPC分类号： C12N504

    CPC分类号： C12N9/0022 ,  A23K10/14 ,  A23K10/18 ,  A23K30/18 ,  C07K2319/00 ,  C12N9/16 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282 ,  G01N2333/906

    摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

    摘要翻译： 本发明提供了从Exophiala spinifera分离的酶APAO的多核苷酸和相关多肽。 此外，编码APAO酶的多核苷酸可用于转化通常易受镰刀菌或其它产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 另外，本发明提供在转基因植物中表达APAO和伏马菌素酯酶。 以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。 此外，本发明提供了在原核和非植物真核系统中生产APAO酶的方法。 还公开了谷物，谷物加工，青贮饲料，粮食作物，动物饲料和瘤胃微生物的解毒方法。

    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    6.
    发明授权
    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 失效
    氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法

    公开(公告)号：US06943279B1

    公开(公告)日：2005-09-13

    申请号：US09658835

    申请日：2000-09-08

    申请人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Aragula Gururaj Rao ,  Oswald R. Crasta ,  Otto Folkerts

    发明人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Aragula Gururaj Rao ,  Oswald R. Crasta ,  Otto Folkerts

    IPC分类号： C12N9/06 ,  C12N15/09 ,  C12N15/31 ,  C12N15/82 ,  A10H1/00 ,  A10H5/10

    CPC分类号： C12N9/0022 ,  C12N15/8242 ,  C12N15/8282

    摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella airovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

    摘要翻译： 本发明提供了从Exophiala spinifera和Rhinocladiella airovirens分离的酶APAO的多核苷酸和相关多肽。 可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。 另外，本发明提供了重组表达盒，宿主细胞，转基因植物和转基因种子。 本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。 此外，本发明提供了在原核和真核系统中生产APAO酶的方法，用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。 还公开了降解植物，谷物，谷物加工，青贮饲料，粮食作物和动物饲料中真菌毒素的方法。

    Defense-related signaling genes and methods of use
    7.
    发明授权
    Defense-related signaling genes and methods of use 失效
    国防相关信号基因和使用方法

    公开(公告)号：US06630615B1

    公开(公告)日：2003-10-07

    申请号：US09640419

    申请日：2000-08-17

    申请人： Dennis L. Bidney ,  Oswald R. Crasta ,  Xu Hu ,  Guihua Lu

    发明人： Dennis L. Bidney ,  Oswald R. Crasta ,  Xu Hu ,  Guihua Lu

    IPC分类号： C12N1509

    CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N9/00 ,  C12N15/8222 ,  C12N15/8226 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/8279 ,  C12N15/8282 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention relates to the genetic manipulation of plants, particularly to the expression of nucleotide sequences from defense-related signaling genes. Isolated nucleotide sequences encoding a neoxanthin cleavage enzyme, an amino acid permease, and a novel protein are provided. Also provided are isolated nucleotide sequences comprising promoters that drive expression in a plant in an inducible or tissue-preferred manner. The nucleotide sequences find use in increasing the resistance of plants to pathogens and other stresses, modifying ABA metabolism in plants, modifying amino acid transport and content in plants, and regulating gene expression in plants. Additionally provided are isolated proteins, and transformed plants and seeds thereof.

    摘要翻译： 本发明涉及植物的遗传操作，特别涉及来自防御相关信号基因的核苷酸序列的表达。 提供了编码新黄素裂解酶，氨基酸通透酶和新型蛋白质的分离的核苷酸序列。 还提供了分离的核苷酸序列，其包含以诱导型或组织优选方式驱动植物表达的启动子。 核苷酸序列用于增加植物对病原体和其他胁迫的抗性，改变植物中的ABA代谢，修饰植物中的氨基酸转运和内容，以及调节植物中的基因表达。 另外提供了分离的蛋白质，以及转化的植物和种子。

    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    8.
    发明授权
    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权
    氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法

    公开(公告)号：US07241934B2

    公开(公告)日：2007-07-10

    申请号：US10743891

    申请日：2003-12-22

    申请人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Aragula Gururaj Rao ,  Oswald R. Crasta ,  Otto Folkerts

    发明人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Aragula Gururaj Rao ,  Oswald R. Crasta ,  Otto Folkerts

    IPC分类号： C12N15/82 ,  C12N15/31 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N9/0022 ,  C12N15/8242 ,  C12N15/8282

    摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

    摘要翻译： 本发明提供了从Exophiala spinifera和Rhinocladiella atvirens分离的酶APAO的多核苷酸和相关多肽。 可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。 另外，本发明提供了重组表达盒，宿主细胞，转基因植物和转基因种子。 本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。 此外，本发明提供了在原核和真核系统中生产APAO酶的方法，用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。 还公开了降解植物，谷物，谷物加工，青贮饲料，粮食作物和动物饲料中真菌毒素的方法。