An operational amplifier includes transistors for providing a controlled current path. At least one of the transistors is in an isolated well in a substrate. Offset of the operational amplifier is corrected by applying a back gate bias voltage to at least one isolated well and changing impedance of the transistors. The proper back gate bias voltage and transistor impedance are determined by incrementally adjusting the back gate bias voltage and then incrementally adjusting the transistor impedance. Calibration values are stored in register memory. Such calibration may be performed by an auto offset calibration process.