发明授权
US06284456B1 Transcriptional coactivator that interacts with Tat protein and regulates its binding to TAR RNA, methods for modulating Tat transactivation, and uses therefor
有权
与Tat蛋白相互作用并调节其与TAR RNA的结合的转录共激活因子，用于调节Tat反式激活的方法及其用途
- 专利标题： Transcriptional coactivator that interacts with Tat protein and regulates its binding to TAR RNA, methods for modulating Tat transactivation, and uses therefor
- 专利标题（中）： 与Tat蛋白相互作用并调节其与TAR RNA的结合的转录共激活因子，用于调节Tat反式激活的方法及其用途
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申请号： US09476482申请日： 1999-12-30
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公开(公告)号： US06284456B1公开(公告)日： 2001-09-04
- 发明人: Katherine A. Jones , Ping Wei , Mitchell Garber , Shi-Min Fang
- 申请人： Katherine A. Jones , Ping Wei , Mitchell Garber , Shi-Min Fang
- 主分类号： C12Q170
- IPC分类号： C12Q170
摘要：
In accordance with the present invention, isolated nucleic acid encoding a host cell protein that regulates Tat transactivation has been discovered. The protein is the first discovered constituent of the TAK/TEFb complex which associates with the HIV Tat, via divalent cation metals, and is necessary for the binding of Tat to TAR RNA. This protein, cyclin T1, is an 87 kDa cyclin partner for the PITALRE kinase. It is further discovered that Tat must interact with TAK in order to bind to TAR RNA with affinity and with the appropriate sequence specificity that is observed in vivo. In accordance with another aspect of the invention, formulations useful for modulation of Tat transactivation have been developed. In addition, assays have been developed for the identification of compounds useful to modulate the above-described processes.