发明授权
US06395880B1 Method for purification of antithrombin III using an anion exchanger
失效
使用阴离子交换剂纯化抗凝血酶III的方法
- 专利标题： Method for purification of antithrombin III using an anion exchanger
- 专利标题（中）： 使用阴离子交换剂纯化抗凝血酶III的方法
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申请号： US09509052申请日： 2000-05-26
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公开(公告)号： US06395880B1公开(公告)日： 2002-05-28
- 发明人: Yendra Linnau , Ernst Hetzl , H. Peter Matthiessen , Silvia Neppl , Wolfgang Schönhofer , Hans-Peter Schwarz
- 申请人： Yendra Linnau , Ernst Hetzl , H. Peter Matthiessen , Silvia Neppl , Wolfgang Schönhofer , Hans-Peter Schwarz
- 优先权： AT1594/97 19970919
- 主分类号： C12N974
- IPC分类号： C12N974
摘要：
A method of purifying antithrombin III (AT III) from a starting material containing an AT III/heparin complex or an AT III/heparinoid complex is disclosed. First, the method comprises adsorbing the AT III/heparin complex or the AT III/heparinoid complex on an anion exchanger material. Second, the method involves separating the AT III from the adsorbed AT III/heparin complex or an AT III/heparinoid complex by elution with a buffer having a pH ranging from 8.5 to 10.5 and a conductivity between 10 and 60 mS.
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