发明授权
US06492121B2 Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
有权
用于确定靶核酸分子的浓度的方法，该方法的核酸探针和用于分析通过该方法得到的数据的方法
- 专利标题： Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
- 专利标题（中）： 用于确定靶核酸分子的浓度的方法，该方法的核酸探针和用于分析通过该方法得到的数据的方法
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申请号： US09725265申请日： 2000-11-29
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公开(公告)号： US06492121B2公开(公告)日： 2002-12-10
- 发明人: Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
- 申请人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
- 优先权： JP11-111601 19990420
- 主分类号： C12Q168
- IPC分类号： C12Q168
摘要：
A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.