公开(公告)号：US06699661B1

公开(公告)日：2004-03-02

申请号：US09556127

申请日：2000-04-20

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

IPC分类号： C12Q168

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2565/549 ,  C12Q2565/107 ,  C12Q2563/107 ,  C12Q2525/185

摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

摘要翻译： 提供了通过使用用荧光染料标记的核酸探针来测定靶核酸的浓度的方法。 该方法包括：提供当与靶核酸杂交时能够降低荧光染料的荧光发射的核酸探针作为探针;将探针与目标核酸杂交; 并测量在杂交之前相对于来自荧光染料的荧光发射的杂交后荧光染料的荧光发射的减少。

公开(公告)号：US06495326B2

公开(公告)日：2002-12-17

申请号：US09725256

申请日：2000-11-29

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

IPC分类号： C12Q168

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2565/549 ,  C12Q2565/107 ,  C12Q2563/107 ,  C12Q2525/185

摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

公开(公告)号：US06492121B2

公开(公告)日：2002-12-10

申请号：US09725265

申请日：2000-11-29

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

IPC分类号： C12Q168

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2565/549 ,  C12Q2565/107 ,  C12Q2563/107 ,  C12Q2525/185

摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

摘要翻译： 提供了通过使用用荧光染料标记的核酸探针来测定靶核酸的浓度的方法。 该方法包括：提供当与靶核酸杂交时能够降低荧光染料的荧光发射的核酸探针作为探针;将探针与目标核酸杂交; 并测量在杂交之前相对于来自荧光染料的荧光发射的杂交后荧光染料的荧光发射的减少。

公开(公告)号：US07094540B2

公开(公告)日：2006-08-22

申请号：US10209608

申请日：2002-08-01

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2565/549 ,  C12Q2565/107 ,  C12Q2563/107 ,  C12Q2525/185

摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

公开(公告)号：US20060177856A1

公开(公告)日：2006-08-10

申请号：US11340574

申请日：2006-01-27

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2565/549 ,  C12Q2565/107 ,  C12Q2563/107 ,  C12Q2525/185

摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

公开(公告)号：US07429655B2

公开(公告)日：2008-09-30

申请号：US10683386

申请日：2003-10-14

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2565/549 ,  C12Q2565/107 ,  C12Q2563/107 ,  C12Q2525/185

摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises:providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid;hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

摘要翻译： 提供了通过使用用荧光染料标记的核酸探针来测定靶核酸的浓度的方法。 该方法包括：当与靶核酸杂交时，提供能够减少来自荧光染料的荧光发射的核酸探针作为探针; 将探针与目标核酸杂交; 并测量在杂交之前相对于来自荧光染料的荧光发射的杂交后荧光染料的荧光发射的减少。

公开(公告)号：US07419786B2

公开(公告)日：2008-09-02

申请号：US11340574

申请日：2006-01-27

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku ,  Osamu Koyama ,  Kenta Furusho

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2565/549 ,  C12Q2565/107 ,  C12Q2563/107 ,  C12Q2525/185

摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

摘要翻译： 提供了通过使用用荧光染料标记的核酸探针来测定靶核酸的浓度的方法。 该方法包括：当与靶核酸杂交时，提供能够减少来自荧光染料的荧光发射的核酸探针作为探针; 将探针与目标核酸杂交; 并测量在杂交之前相对于来自荧光染料的荧光发射的杂交后荧光染料的荧光发射的减少。

公开(公告)号：US07273700B2

公开(公告)日：2007-09-25

申请号：US10399407

申请日：2002-03-27

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： G01N33/582 ,  C12Q1/6818 ,  C12Q2565/101 ,  C12Q2565/107 ,  C12Q2565/1015

摘要： A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.

摘要翻译： 用于核酸测定的新型核酸探针包括用含有至少一对荧光染料的多种荧光染料标记以诱导FRET的单链核酸，该对荧光染料包括能够服用的荧光染料（供体染料） 作为能够作为受体染料的供体染料和荧光染料（受体染料），其中核酸探针具有这样的碱基序列并用荧光染料标记，使得受体染料的荧光强度降低 与靶核酸杂交。 一种新型核酸测定方法使用探针。 探针和方法可以使用简单的装置并行测定测定系统中的一种或多种类型的靶核酸。

公开(公告)号：US20050048485A1

公开(公告)日：2005-03-03

申请号：US10399407

申请日：2002-03-27

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

IPC分类号： C12Q1/68 ,  G01N33/58 ,  C07H21/04

CPC分类号： G01N33/582 ,  C12Q1/6818 ,  C12Q2565/101 ,  C12Q2565/107 ,  C12Q2565/1015

摘要： A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.

摘要翻译： 用于核酸测定的新型核酸探针包括用含有至少一对荧光染料的多种荧光染料标记以诱导FRET的单链核酸，该对荧光染料包括能够服用的荧光染料（供体染料） 作为能够用作受体染料的供体染料和荧光染料（受体染料），其中核酸探针具有这样的碱基序列并用荧光染料标记，使得受体染料的荧光强度降低 与靶核酸杂交。 一种新型核酸测定方法使用探针。 探针和方法可以使用简单的装置并行测定测定系统中的一种或多种类型的靶核酸。

公开(公告)号：US07354707B2

公开(公告)日：2008-04-08

申请号：US09891517

申请日：2001-06-27

申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

IPC分类号： C12Q1/68 ,  C07H21/00 ,  G06F19/00

CPC分类号： C12Q1/6818 ,  C12Q1/6851 ,  Y02A90/26 ,  C12Q2565/107 ,  C12Q2565/1015

摘要： Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.

摘要翻译： 提供核酸探针，每个核酸探针由可与靶核酸杂交并用荧光染料或荧光染料和猝灭剂物质标记的单链寡核苷酸形成。 核酸探针可以在短时间内容易地设计，允许测定，多形态分析或核酸的实时定量PCR，并且在反应期间不解离。 还提供了使用核酸探针的核酸测定方法，多晶分析方法和实时定量PCR方法。