发明授权
- 专利标题: Methods for producing members of specific binding pairs
- 专利标题(中): 生产特异性结合对成员的方法
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申请号: US09104337申请日: 1998-06-25
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公开(公告)号: US06492160B1公开(公告)日: 2002-12-10
- 发明人: Andrew David Griffiths , Samuel Cameron Williams , Peter Michael Waterhouse , Ahuva Nissim , Gregory Paul Winter , Kevin Stuart Johnson , Andrew John Hammond Smith
- 申请人: Andrew David Griffiths , Samuel Cameron Williams , Peter Michael Waterhouse , Ahuva Nissim , Gregory Paul Winter , Kevin Stuart Johnson , Andrew John Hammond Smith
- 优先权: GB9110549 19910515; GB9206318 19920324
- 主分类号: C12N701
- IPC分类号: C12N701
摘要:
Methods, recombinant host cells and kits are disclosed-for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of a sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.
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