利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

32 条记录 (耗时 0.018 秒)

    EHV-1 vectors
    1.
    发明授权
    EHV-1 vectors 失效
    EHV-1载体

    公开(公告)号：US06706515B2

    公开(公告)日：2004-03-16

    申请号：US10100548

    申请日：2002-03-18

    申请人： Alexander Fred Markham ,  David Mark Meredith

    发明人： Alexander Fred Markham ,  David Mark Meredith

    IPC分类号： C12N701

    CPC分类号： C12N15/86 ,  C12N2710/16721 ,  C12N2710/16743

    摘要： The invention relates to manipulation of equine herpes virus for use in gene therapy and in particular to modifications of the virus so that it can carry heterologous material and furthermore, preferably, be replication deficient so that the virus cannot replicate in the target tissue.

    摘要翻译： 本发明涉及用于基因治疗的马疱疹病毒的操作，特别是涉及病毒的修饰以使其能够携带异源物质，此外，优选为复制缺陷型，使得病毒不能在靶组织中复制。

    Materials and methods for simplified AAV production
    5.
    发明授权
    Materials and methods for simplified AAV production 失效
    简化AAV生产的材料和方法

    公开(公告)号：US06221646B1

    公开(公告)日：2001-04-24

    申请号：US09127268

    申请日：1998-07-31

    申请人： Varavani Dwarki ,  Martha Baillie Ladner ,  Jaime Escobedo ,  Shang-Zhen Zhou

    发明人： Varavani Dwarki ,  Martha Baillie Ladner ,  Jaime Escobedo ,  Shang-Zhen Zhou

    IPC分类号： C12N701

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2750/14143 ,  C12N2750/14162

    摘要： The invention provides a method for producing purified replication-defective recombinant AAV virions. The method comprises introducing into a suitable host cell an AAV vector, an AAV helper construct and an adenoplasmid accessory construct into the host cell. The adenoplasmid accessory plasmid is composed adenovirus plasmid DNA unable to be packaged into adenoviral particles because it lacks packaging signal sequence(s) or it contains additional sequences making it too large to package. The host cell is cultured to produce crude rAAV virions and then lysed. The resulting cell lysate is applied to a chromatographic column containing sulfonated cellulose or subjected to cesium chloride equilibrium gradient centrifugation and the purified rAAV virions are recovered.

    摘要翻译： 本发明提供了一种用于产生纯化的复制缺陷型重组AAV病毒体的方法。 该方法包括向宿主细胞中引入AAV载体，AAV辅助构建体和腺瘤辅助构建体的合适宿主细胞。 腺病毒质粒辅助质粒是由于缺乏包装信号序列而导致的腺病毒质粒DNA无法包装，因为它包含其它序列使其太大而不能包装。 培养宿主细胞以产生粗rAAV病毒粒子，然后裂解。 将得到的细胞裂解物应用于含有磺化纤维素的色谱柱或进行氯化铯平衡梯度离心，并回收纯化的rAAV病毒粒子。

    Toxin-phage bacteriocide antibiotic and uses thereof
    8.
    发明授权
    Toxin-phage bacteriocide antibiotic and uses thereof 失效
    毒素噬菌体杀菌剂抗生素及其用途

    公开(公告)号：US06759229B2

    公开(公告)日：2004-07-06

    申请号：US10025598

    申请日：2001-12-18

    申请人： Diane L. Schaak

    发明人： Diane L. Schaak

    IPC分类号： C12N701

    CPC分类号： C12N15/86 ,  A61K35/76 ,  A61K38/16 ,  C07K14/005 ,  C12N7/00 ,  C12N15/70 ,  C12N15/74 ,  C12N2710/10321 ,  C12N2710/10332 ,  C12N2710/10344 ,  C12N2750/14143 ,  A61K2300/00

    摘要： The invention provides intracellular peptide toxins capable of killing bacterial and eukaryotic cells when present within the cell, while substantially lacking the ability to kill such cells when present externally. The invention also provides recombinant bacteriophage containing nucleic acid sequences encoding intracellular peptide toxins, and methods of using such bacteriophage to kill bacteria. Furthermore, the invention provides compositions, including pharmaceutical compositions, which can be used to kill bacteria or inhibit the growth of bacteria both in vitro and in vivo. Methods of treating a bacterial infection in a subject are also provided by the invention.

    摘要翻译： 本发明提供了当存在于细胞内时能够杀死细菌和真核细胞的细胞内肽毒素，而当外部存在时基本上缺乏杀死这种细胞的能力。 本发明还提供了含有编码细胞内肽毒素的核酸序列的重组噬菌体，以及使用这种噬菌体杀死细菌的方法。 此外，本发明提供了可用于在体外和体内杀死细菌或抑制细菌生长的组合物，包括药物组合物。 治疗受试者细菌感染的方法也由本发明提供。

    Method for enhanced protein stabilization and for production of cell lines useful for production of such stabilized proteins
    9.
    发明授权
    Method for enhanced protein stabilization and for production of cell lines useful for production of such stabilized proteins 有权
    增强蛋白质稳定化和生产可用于生产这种稳定化蛋白质的细胞系的方法

    公开(公告)号：US06468777B2

    公开(公告)日：2002-10-22

    申请号：US09803634

    申请日：2001-03-09

    申请人： H. Craig Dees ,  John Smolik

    发明人： H. Craig Dees ,  John Smolik

    IPC分类号： C12N701

    CPC分类号： C07K14/47 ,  C12N7/00 ,  C12N2760/18451

    摘要： A method for increasing production yield of viruses, viral proteins, and other related biological materials through enhanced control and stabilization of protein production via stress proteins and the resultant protein products. The present invention is also directed to methods for selection or engineering of cell lines yielding such enhanced stabilized products. More specifically, example embodiments of the present invention are directed to methods for enhancing production of a viral agent, production of cell lines exhibiting permanent genetic modification, production of permissive eucaryotic cell lines, enhancing functional recombinant product yield, and the products of such methods.

    摘要翻译： 通过应激蛋白和产生的蛋白质产物增强蛋白质生产的控制和稳定来增加病毒，病毒蛋白质和其他相关生物材料的产量的方法。 本发明还涉及用于选择或工程化产生这种增强的稳定化产物的细胞系的方法。 更具体地，本发明的示例性实施方案涉及用于增强病毒剂的产生，显示永久遗传修饰的细胞系的产生，允许的真核细胞系的产生，增强功能重组产物产量以及这些方法的产物的方法。

    Viral nucleotide sequences
    10.
    发明授权
    Viral nucleotide sequences 失效
    病毒核苷酸序列

    公开(公告)号：US06413762B2

    公开(公告)日：2002-07-02

    申请号：US09745810

    申请日：2000-12-26

    申请人： Annette Mary Griffin ,  Louis Joseph Norman Ross ,  Simon David Scott ,  Matthew McKinley Binns

    发明人： Annette Mary Griffin ,  Louis Joseph Norman Ross ,  Simon David Scott ,  Matthew McKinley Binns

    IPC分类号： C12N701

    CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K39/255 ,  A61K2039/51 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N9/0093 ,  C12N9/1211 ,  C12N15/00 ,  C12N2710/16022 ,  C12N2710/16034 ,  C12N2710/16043 ,  C12N2710/16322 ,  C12N2710/16334 ,  C12N2710/16343

    摘要： Various genes of herpes virus of turkeys (HVT) Marek's disease virus (MDV) and infectious laryngotracheitis virus (ILTV) have been identified as non-essential regions (and candidates for insertion sites for foreign genes) and/or as antigen-encoding regions. The former include the HVT homologue of the HSV (herpes simplex virus) gC gene, the TK (thymidine kinase) region of MDV or ILTV, ORF3 of ILTV (as defined herein), the ribonucleotide reductase (large subunit) gene of ILTV, MDV or HVT and the ribonucleotide reductase (small subunit) gene of MDV. The antigen-encoding regions include the HVT homologues of the HSV gB, gC and gH genes, the ILTV homologue of HSV gB, ORF2 of ILTV, and the HVT homologue of the HSV-1 immediate early genes IE-175 and IE-68. Manipulation of these genes allows vaccines to be prepared comprising attenuated virus or virus carrying heterologous antigen-encoding sequences.

    摘要翻译： 火鸡疱疹病毒（HVT）马立克病病毒（MDV）和感染性喉气管炎病毒（ILTV）的各种基因已被鉴定为非必需区域（以及外源基因插入位点的候选者）和/或作为抗原编码区域。 前者包括HSV（单纯疱疹病毒）gC基因的HVT同源物，MDV或ILTV的TK（胸苷激酶）区，ILTV的ORF3（如本文所定义），ILTV的核糖核苷酸还原酶（大亚基）基因，MDV 或HVT和MDV的核糖核苷酸还原酶（小亚基）基因。 抗原编码区包括HSV gB，gC和gH基因的HVT同源物，HSV gB的ILTV同源物，ILTV的ORF2和HSV-1即时早期基因IE-175和IE-68的HVT同源物。 这些基因的操作允许制备包含减毒病毒或携带异源抗原编码序列的病毒的疫苗。