发明授权
- 专利标题: Screening of phage displayed peptides without clearing of the cell culture
- 专利标题(中): 筛选噬菌体展示肽,而不清除细胞培养
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申请号: US09874547申请日: 2001-06-04
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公开(公告)号: US06686154B2公开(公告)日: 2004-02-03
- 发明人: Steffen Nock , Paul D. Kassner
- 申请人: Steffen Nock , Paul D. Kassner
- 主分类号: C12Q168
- IPC分类号: C12Q168
摘要:
This invention provides methods for screening populations of phage-displayed polypeptides that are particularly well-suited for high-throughput screening. The methods do not require the clearing of cells from a culture used to obtain the population of phage or other replicable genetic packages. Accordingly, the invention provides methods for forming complexes between a replicable genetic package displaying a polypeptide fusion and a target molecule in an uncleared cell culture containing replicable genetic package. Compositions made up of an uncleared cell culture containing replicable genetic packages displaying a polypeptide fusion and a target molecule are provided in the invention as well.