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    MICRODEVICES FOR HIGH-THROUGHPUT SCREENING OF BIOMOLECULES
    2.
    发明申请
    MICRODEVICES FOR HIGH-THROUGHPUT SCREENING OF BIOMOLECULES 审中-公开
    用于生物分子高通量筛选的MICRODEVICES

    公开(公告)号：US20090047695A1

    公开(公告)日：2009-02-19

    申请号：US12173515

    申请日：2008-07-15

    申请人： Peter Wagner ,  Dana Ault-Riche ,  Steffen Nock ,  Christian Itin

    发明人： Peter Wagner ,  Dana Ault-Riche ,  Steffen Nock ,  Christian Itin

    IPC分类号： C12Q1/48 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543

    CPC分类号： B82Y30/00 ,  Y10S435/805 ,  Y10S435/81 ,  Y10T436/143333

    摘要： Methods and devices for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules that can be immobilized on the surface of the devices of the present invention include proteins, polypeptides, nucleic acids, polysaccharides, phospholipids, and related unnatural polymers of biological relevance. These devices are useful in high-throughput drug screening and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.

    摘要翻译： 提供了使用微型微加工装置并行，体外筛选生物分子活性的方法和装置。 可以固定在本发明装置表面上的生物分子包括具有生物相关性的蛋白质，多肽，核酸，多糖，磷脂和相关的非天然聚合物。 这些装置在高通量药物筛选和临床诊断中是有用的，并且优选用于相关蛋白家族的并行筛选。

    Microdevices for screening biomolecules
    4.
    发明授权
    Microdevices for screening biomolecules 失效
    用于筛选生物分子的微型装置

    公开(公告)号：US06682942B1

    公开(公告)日：2004-01-27

    申请号：US09570589

    申请日：2000-05-12

    申请人： Peter Wagner ,  Dana Ault-Riche ,  Steffen Nock ,  Christian Itin

    发明人： Peter Wagner ,  Dana Ault-Riche ,  Steffen Nock ,  Christian Itin

    IPC分类号： G01N33543

    CPC分类号： B82Y30/00

    摘要： Methods for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules immobilized on the surface of the devices employed in the method of the present invention include proteins, polypeptides, polynucleotides, polysaccharides, phospholipids, and related unnatural polymers of biological relevance. These methods are useful in drug development, functional proteomics studies and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.

    摘要翻译： 提供了使用微型微型加工装置平行体外筛选生物分子活性的方法。 固定在本发明方法中使用的装置表面上的生物分子包括具有生物相关性的蛋白质，多肽，多核苷酸，多糖，磷脂和相关的非天然聚合物。 这些方法可用于药物开发，功能蛋白质组学研究和临床诊断，并且优选用于相关蛋白家族的平行筛选。

    Super-humanized antibodies against respiratory syncytial virus
    6.
    发明申请
    Super-humanized antibodies against respiratory syncytial virus 审中-公开
    针对呼吸道合胞病毒的超人源化抗体

    公开(公告)号：US20050288491A1

    公开(公告)日：2005-12-29

    申请号：US11061848

    申请日：2005-02-17

    申请人： David Wilson ,  Steffen Nock ,  James Larrick

    发明人： David Wilson ,  Steffen Nock ,  James Larrick

    IPC分类号： A61K39/42 ,  C07K16/10 ,  C07K16/44

    CPC分类号： C07K16/1027 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565

    摘要： Disclosed herein are humanized antibodies that bind to an epitope on the F protein of respiratory syncytial virus. The humanized antibodies were designed by comparing the canonical CDR structure types of the CDRs from a non-human antibody (HNK20) to the canonical CDR structure types found in the human antibody germline sequences as the basis for selecting human variable region frameworks in a method denoted “super-humanization.” Human antibody variable regions having the same or similar canonical CDR structure types as the non-human CDR provided a subset of candidate sequences from which to select the human frameworks. Chimeric variable regions were made comprising the non-human CDRs grafted in corresponding locations into the human frameworks from the candidate human variable regions. Several humanized antibodies that bind the same antigen as HNK20 and that have low immunogenicity were thereby designed, including examples where the framework sequences have less than 65% amino acid identity to the non-human frameworks.

    摘要翻译： 本文公开了与呼吸道合胞病毒的F蛋白上的表位结合的人源化抗体。 通过将来自非人抗体（HNK20）的CDR的规范CDR结构类型与在人抗体种系序列中发现的规范CDR结构类型进行比较来设计人源化抗体，作为在所述方法中选择人可变区框架的基础 “超人性化”。 具有与非人CDR相同或相似的典型CDR结构类型的人抗体可变区提供了选择人骨架的候选序列的子集。 制备嵌合可变区，其包含从候选人可变区移植到人框架中的相应位置的非人CDR。 由此设计了结合与HNK20相同的抗原并且具有低免疫原性的几种人源化抗体，包括其中框架序列与非人框架具有小于65％氨基酸同一性的实例。

    Display of dimeric proteins on phage
    7.
    发明申请
    Display of dimeric proteins on phage 审中-公开
    在噬菌体上显示二聚体蛋白质

    公开(公告)号：US20050147962A1

    公开(公告)日：2005-07-07

    申请号：US10717735

    申请日：2003-11-19

    申请人： Christopher Wagstrom ,  David Wilson ,  Steffen Nock

    发明人： Christopher Wagstrom ,  David Wilson ,  Steffen Nock

    IPC分类号： C07K16/00 ,  C12Q1/70 ,  C07H21/04 ,  C07K16/18 ,  C12N1/21 ,  C12N15/74

    CPC分类号： C07K16/005

    摘要： Expression vectors for expressing multimeric polypeptides that are anchored on surfaces of genetically replicable packages are disclosed. The expression vectors include a vector segment encoding a polypeptide sequence having three polypeptide segments. One of the segments contains a cleavable peptide sequence cleavable by a proteolytic agent, and another segment has an anchoring peptide sequence for anchoring the multimeric polypeptide to the surface of the genetically replicable package. The cleavable peptide sequence is cleaved by the proteolytic agent and the first segment associates with the third segment to form the multimeric polypeptide. Also disclosed are methods, host cells, and kits employing the expression vectors.

    摘要翻译： 公开了用于表达锚定在遗传可复制包装表面上的多聚体多肽的表达载体。 表达载体包括编码具有三个多肽片段的多肽序列的载体片段。 其中一个片段含有可由蛋白水解酶切割的可切割肽序列，另一个片段具有用于将多聚体多肽锚定于基因可复制包装表面的锚定肽序列。 可切割的肽序列被蛋白水解酶切割，第一个片段与第三个片段缔合以形成多聚体多肽。 还公开了使用表达载体的方法，宿主细胞和试剂盒。