发明授权
- 专利标题: Methods and apparatus for electrophoresis of prior-cast, hydratable separation media
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申请号: US10102188申请日: 2002-03-18
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公开(公告)号: US06936150B2公开(公告)日: 2005-08-30
- 发明人: Regina D. Rooney , Bradley S. Scott , Joseph W. Amshey , Thomas R. Jackson , Sheldon Engelhorn
- 申请人: Regina D. Rooney , Bradley S. Scott , Joseph W. Amshey , Thomas R. Jackson , Sheldon Engelhorn
- 申请人地址: US CA Carlsbad
- 专利权人: Invitrogen Corporation
- 当前专利权人: Invitrogen Corporation
- 当前专利权人地址: US CA Carlsbad
- 代理机构: Fish & Neave IP Group Ropes & Gray LLP
- 代理商 James F. Haley, Jr.; Tae Bum Shin
- 主分类号: B01D57/02
- IPC分类号: B01D57/02 ; B03C5/00 ; C07K1/26 ; C08F2/58 ; G01N27/447 ; G01N33/559 ; G01N33/561 ; G01N27/453
摘要:
Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.