- 专利标题： Method for amplifying nucleic acid sequence
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申请号： US09935338申请日： 2001-08-23
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公开(公告)号： US06951722B2公开(公告)日： 2005-10-04
- 发明人: Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
- 申请人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
- 申请人地址： JP Shiga
- 专利权人： Takara Bio Inc.
- 当前专利权人： Takara Bio Inc.
- 当前专利权人地址： JP Shiga
- 代理机构： Browdy and Neimark, PLLC
- 优先权： JP11-076966 19990319; JP11-370035 19991227; JP2000-251981 20000823; JP2000-284419 20000919; JP2000-288750 20000922; JP2001-104191 20010403
- 主分类号： C07H21/04
- IPC分类号： C07H21/04 ; C12N9/22 ; C12P19/34 ; C12Q1/68
摘要：
A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
公开/授权文献
- US20030073081A1 Method for amplifying nucleic acid sequence 公开/授权日：2003-04-17
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