发明授权
- 专利标题： Prolidase and its gene and method for producing prolidase
- 专利标题（中）： 脯氨酸酶及其基因及产生脯氨酸酶的方法
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申请号： US10325939申请日： 2002-12-23
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公开(公告)号： US06951750B2公开(公告)日： 2005-10-04
- 发明人: Kotaro Ito , Takeharu Nakahara , Yasuji Koyama , Toshifumi Matsuda , Tadashi Takahashi , Kenichiro Matsushima , Genryou Umitsuki , Tsutomu Masuda
- 申请人： Kotaro Ito , Takeharu Nakahara , Yasuji Koyama , Toshifumi Matsuda , Tadashi Takahashi , Kenichiro Matsushima , Genryou Umitsuki , Tsutomu Masuda
- 申请人地址： JP Noda JP Noda
- 专利权人： Kikkoman Corporation,Noda Institute for Scientific Research
- 当前专利权人： Kikkoman Corporation,Noda Institute for Scientific Research
- 当前专利权人地址： JP Noda JP Noda
- 代理机构： Oblon, Spivak, McClelland, Maier & Neustadt, P.C.
- 优先权： JP2001-395306 20011226; JP2002-283759 20020927
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N1/15 ; C12N1/19 ; C12N1/21 ; C12N5/10 ; C12N9/48 ; C12N9/62 ; C12N15/57
摘要：
Provided are a protein comprising the amino acid sequence as shown in SEQ ID NO: 2 and having prolidase activities, a prolidase gene coding for the protein, a prolidase gene comprising the nucleotide sequence as shown in SEQ ID NO: 1, recombinant DNA having the gene inserted into vector DNA, and a transformant or transductant comprising the recombinant DNA, and a method for producing prolidase using the transformant or transductant.The present invention can improve the prolidase through protein engineering. The present invention can be also used for improving microorganisms used in the production of enzymes for food processing and fermented foods.
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