发明授权
US07011958B2 Method for improving the stability of linear DNA in cell-free in vitro transcription/translation systems
失效
提高无细胞体外转录/翻译系统中线性DNA稳定性的方法
- 专利标题: Method for improving the stability of linear DNA in cell-free in vitro transcription/translation systems
- 专利标题(中): 提高无细胞体外转录/翻译系统中线性DNA稳定性的方法
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申请号: US10124663申请日: 2002-04-17
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公开(公告)号: US07011958B2公开(公告)日: 2006-03-14
- 发明人: Manfred Watzele , Thomas Hoffmann , Cordula Nemetz , Dieter Heindl , Thomas Metzler , Wolfgang Mutter
- 申请人: Manfred Watzele , Thomas Hoffmann , Cordula Nemetz , Dieter Heindl , Thomas Metzler , Wolfgang Mutter
- 申请人地址: US IN Indianapolis
- 专利权人: Roche Diagnostics Operations, Inc.
- 当前专利权人: Roche Diagnostics Operations, Inc.
- 当前专利权人地址: US IN Indianapolis
- 代理机构: Roche Diagnostics Operations, Inc.
- 代理商 Marilyn L. Amick
- 优先权: DE10119006 20010418
- 主分类号: C12P21/06
- IPC分类号: C12P21/06 ; C12P19/34
摘要:
The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.
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