摘要:
The present invention concerns a matrix reactor for reactions in which low-molecular and high-molecular reactants are involved wherein the reactor space VR contains a matrix and comprises a reaction compartment and a supply compartment, wherein the matrix is composed of a porous material which can take up low-molecular reactants and wherein the exclusion volume V0 of the matrix is available as the reaction compartment and the matrix volume VM of the matrix is available as the supply compartment. The matrix reactor can be used for enzymatic processes, in particular for a coupled in vitro transcription/translation reaction to produce proteins.
摘要翻译:本发明涉及用于其中涉及低分子和高分子反应物的反应的基质反应器,其中反应器空间V R R含有基质并且包含反应室和供应室,其中基质 由可以吸收低分子量反应物的多孔材料组成,并且其中基质的排阻体积V 0 O可用作反应室,并且基体体积V M 的矩阵可用作供应隔室。 基质反应器可用于酶法,特别是用于偶联的体外转录/翻译反应以产生蛋白质。
摘要:
The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.
摘要:
The invention concerns a method for the determination of an analyte in a sample liquid wherein the sample liquid is incubated with (a) a first receptor which binds to a first epitope that is only present once on the analyte and is immobilized on a particulate carrier material and (b) a second receptor which binds to a second epitope on the analyte and has at least two binding sites for the second epitope, the first epitope being different from the second epitope and the analyte is determined by means of the binding to both receptors.
摘要:
The use of polyalkylene oxide modified reagents is disclosed in a method for the detection of an analyte or in suitable reagent kits for such methods.