Invention Grant
US07056672B2 Method of identifying an open reading frame using a nucleic acid molecule encoding multiple start codons and histidine tags
失效
使用编码多个起始密码子和组氨酸标签的核酸分子鉴定开放阅读框的方法
- Patent Title: Method of identifying an open reading frame using a nucleic acid molecule encoding multiple start codons and histidine tags
- Patent Title (中): 使用编码多个起始密码子和组氨酸标签的核酸分子鉴定开放阅读框的方法
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Application No.: US10766102Application Date: 2004-01-27
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Publication No.: US07056672B2Publication Date: 2006-06-06
- Inventor: Patrick S. Schnable , Feng Liu , Yan Fu
- Applicant: Patrick S. Schnable , Feng Liu , Yan Fu
- Applicant Address: US IA Ames
- Assignee: Iowa State University Research Foundation, Inc.
- Current Assignee: Iowa State University Research Foundation, Inc.
- Current Assignee Address: US IA Ames
- Agency: Fish & Richardson P.C., P.A.
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P21/00 ; C12P21/02
Abstract:
Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5′ or 3′ to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3′ of the ATG start codon and 5′ of the 3-frame His-tag DNA sequence.
Public/Granted literature
- US20050048523A1 Nucleic acid molecules encoding multiple start codons and histidine tags Public/Granted day:2005-03-03
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