发明授权
US07288372B2 Methods for the preparation of chemically misaminoacylated tRNA via protective groups
有权
通过保护基制备化学失氨基酰化tRNA的方法
- 专利标题： Methods for the preparation of chemically misaminoacylated tRNA via protective groups
- 专利标题（中）： 通过保护基制备化学失氨基酰化tRNA的方法
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申请号： US10345664申请日： 2003-01-16
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公开(公告)号： US07288372B2公开(公告)日： 2007-10-30
- 发明人: Jerzy Olejnik , Edyta Krzymanska-Olejnik , Sergey Mamaev , Kenneth J. Rothschild
- 申请人： Jerzy Olejnik , Edyta Krzymanska-Olejnik , Sergey Mamaev , Kenneth J. Rothschild
- 申请人地址： US MA Waltham
- 专利权人： Ambergen, Inc.
- 当前专利权人： Ambergen, Inc.
- 当前专利权人地址： US MA Waltham
- 代理机构： Medlen & Carroll, LLP
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; G01N33/00 ; G01N33/53 ; C07K14/00 ; C07H21/02
摘要：
The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.