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    PHOTOCLEAVABLE MASS-TAGS FOR MULTIPLEXED MASS SPECTROMETRIC IMAGING OF TISSUES USING BIOMOLECULAR PROBES
    2.
    发明申请
    PHOTOCLEAVABLE MASS-TAGS FOR MULTIPLEXED MASS SPECTROMETRIC IMAGING OF TISSUES USING BIOMOLECULAR PROBES 有权

    公开(公告)号：US20220137066A1

    公开(公告)日：2022-05-05

    申请号：US17399434

    申请日：2021-08-11

    申请人： AmberGen, Inc.

    发明人： Mark J. Lim ,  Gargey Yagnik ,  Kenneth J. Rothschild

    IPC分类号： G01N33/68

    摘要： The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands. Finally, the field of the invention also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI, such as direct label-free MSI of endogenous biomolecules from the biospecimen (e.g., tissue), whereby said biomolecules can be intact or digested (e.g., chemically digested or by enzyme).

    NOVEL PHOTOCLEAVABLE MASS-TAGS FOR MULTIPLEXED MASS SPECTROMETRIC IMAGING OF TISSUES USING BIOMOLECULAR PROBES
    3.
    发明申请
    NOVEL PHOTOCLEAVABLE MASS-TAGS FOR MULTIPLEXED MASS SPECTROMETRIC IMAGING OF TISSUES USING BIOMOLECULAR PROBES 有权

    公开(公告)号：US20220135518A1

    公开(公告)日：2022-05-05

    申请号：US17399461

    申请日：2021-08-11

    申请人： AmberGen, Inc.

    发明人： Mark J. Lim ,  Gargey Yagnik ,  Kenneth J. Rothschild

    IPC分类号： C07C271/20 ,  G01N33/68

    摘要： The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands. Finally, the field of the invention also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI, such as direct label-free MSI of endogenous biomolecules from the biospecimen (e.g., tissue), whereby said biomolecules can be intact or digested (e.g., chemically digested or by enzyme).

    Methods and compositions for phototransfer
    4.
    发明授权
    Methods and compositions for phototransfer 有权
    光转染的方法和组合物

    公开(公告)号：US08906700B2

    公开(公告)日：2014-12-09

    申请号：US13553472

    申请日：2012-07-19

    申请人： Mark J. Lim ,  Kenneth J. Rothschild

    发明人： Mark J. Lim ,  Kenneth J. Rothschild

    IPC分类号： G01N33/543 ,  G01N33/53 ,  C40B30/04

    CPC分类号： G01N33/54306 ,  C12Q1/6806 ,  G01N33/54353 ,  G01N33/54393

    摘要： Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.

    摘要翻译： 描述了将化合物从第一表面转移到第二表面的方法。 用可光洁化的接头描述化合物。 通过光可透洗接头连接到第一表面的化合物与第二表面接近（或接触），然后在暴露于电磁辐射时转移到第二表面。 用辐射照射化合物使化合物从第一表面光化，并将化合物转移到第二表面。

    Amplifying bisulfite-treated template
    5.
    发明申请
    Amplifying bisulfite-treated template 有权
    扩增亚硫酸氢盐处理的模板

    公开(公告)号：US20100311042A1

    公开(公告)日：2010-12-09

    申请号：US12291178

    申请日：2008-11-06

    申请人： Mark J. Lim ,  Kenneth J. Rothschild

    发明人： Mark J. Lim ,  Kenneth J. Rothschild

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12P19/34 ,  C12Q1/6844

    摘要： Methods of amplifying nucleic acid are described. Primers on a solid support, e.g. a population of beads, are employed. A population of nucleic acid template molecules, wherein said nucleic acid template molecules have been treated with bisulfite, is amplified so as to create loaded beads comprising immobilized amplified nucleic acid.

    摘要翻译： 描述了扩增核酸的方法。 固体支持物上的引物，例如 一群珠子被雇用。 扩增核酸模板分子群体，其中所述核酸模板分子已经用亚硫酸氢盐处理，以产生包含固定化扩增核酸的负载珠。

    Methods for the preparation of chemically misaminoacylated tRNA via protective groups
    9.
    发明授权
    Methods for the preparation of chemically misaminoacylated tRNA via protective groups 有权
    通过保护基制备化学失氨基酰化tRNA的方法

    公开(公告)号：US07524941B2

    公开(公告)日：2009-04-28

    申请号：US11900323

    申请日：2007-09-11

    申请人： Jerzy Olejnik ,  Edyta Krzymanska-Olejnik ,  Sergey Mamaev ,  Kenneth J. Rothschild

    发明人： Jerzy Olejnik ,  Edyta Krzymanska-Olejnik ,  Sergey Mamaev ,  Kenneth J. Rothschild

    IPC分类号： C07C245/00 ,  C07C291/00 ,  C12Q1/68 ,  C07K14/00 ,  G01N33/00 ,  C07H21/04

    CPC分类号： C07H21/02 ,  C07H21/04 ,  G01N33/532 ,  Y02P20/55

    摘要： The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.

    摘要翻译： 本发明涉及用于制备化学氨基酰化tRNA以便将标记物引入新生蛋白质的方法。 本发明还涉及在使用化学氨酰化tRNA的细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物。 标记可以包含可切割部分，可检测标记，报告基因特性，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区别。

    Photocleavable isotope-coded affinity tags
    10.
    发明申请
    Photocleavable isotope-coded affinity tags 失效
    可透光同位素编码的亲和标签

    公开(公告)号：US20080032417A1

    公开(公告)日：2008-02-07

    申请号：US11582294

    申请日：2006-10-17

    申请人： Jerzy Olejnik ,  Kenneth Rothschild

    发明人： Jerzy Olejnik ,  Kenneth Rothschild

    IPC分类号： C07D495/04 ,  C07K1/00 ,  G01N33/68

    CPC分类号： G01N33/532 ,  C07D495/04 ,  C07K1/1077 ,  C07K1/13 ,  C07K1/14 ,  C07K1/16 ,  C07K1/22 ,  G01N2458/15 ,  Y10T436/24

    摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as proteins and protein fragments from biological samples from in vivo and in vitro sources. Agents comprise a detectable group bound to a photoreactive group. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals.

    摘要翻译： 本发明涉及可用于从体内和体外来源的生物样品的复杂混合物如蛋白质和蛋白质片段检测和分离靶组分的试剂和缀合物。 试剂包含与光反应性基团结合的可检测基团。 缀合物包括通过共价界限偶联到底物的试剂，其可以通过施用电磁辐射被选择性地切割。 目标可以用可检测分子标记的物质可以很容易地识别，并与物质的异源混合物分离。 缀合物暴露于放射线以功能形式释放靶，完全不变。 使用可光致发光的分子前体作为共轭物，标记可以并入大分子中，分离出新生大分子，并将标记完全除去。 本发明还涉及用这些缀合物分离的靶，其可用作可以施用于人和其它哺乳动物的药剂或组合物。