发明授权
- 专利标题: Rumen bacteria variants and process for preparing succinic acid employing the same
- 专利标题(中): 瘤胃细菌变异体及使用其制备琥珀酸的方法
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申请号: US10580556申请日: 2004-05-20
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公开(公告)号: US07470530B2公开(公告)日: 2008-12-30
- 发明人: Sang Yup Lee , Sang Jun Lee
- 申请人: Sang Yup Lee , Sang Jun Lee
- 申请人地址: KR Daejeon
- 专利权人: Korea Advanced Institute of Science and Technology
- 当前专利权人: Korea Advanced Institute of Science and Technology
- 当前专利权人地址: KR Daejeon
- 代理机构: Intellectual Property/ Technology Law
- 代理商 Kelly Reynolds; Steven J. Hultquist
- 优先权: KR10-2003-0084934 20031127; KR10-2004-0028105 20040423
- 国际申请: PCT/KR2004/001210 WO 20040520
- 国际公布: WO2005/052135 WO 20050609
- 主分类号: C12N1/20
- IPC分类号: C12N1/20 ; C12N1/12 ; C12N9/10 ; C12P7/46
摘要:
Provided are novel rumen bacterial mutants resulted from the disruption of a lactate dehydrogenase gene (ldhA) and a pyruvate formate-lyase gene (pfl) from rumen bacteria; a novel bacterial mutant (Mannheimia sp. LPK7) having disruptions of a ldhA, a pfl,a phosphotransacetylase gene (pta), and a acetate kinase gene (ackA); a novel bacterial mutant (Mannheimia sp. LPK4) having disruptions of a ldhA, a pfl, and a phosphoenolpyruvate carboxylase gene (ppc) involved in the immobilization of CO2 in a metabolic pathway of producing succinic acid; and a method for producing succinic acid, characterized by culture of the above mutants in anaerobic conditions. The bacterial mutants have the property of producing succinic acid at high concentration while producing little or no organic acids, as compared to the prior wild-type strains of producing various organic acids. Thus, the bacterial mutants are useful as strains for the industrial production of succinic acid.
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