A seal assembly adapted for use in conjunction with a trocar assembly includes a plurality of seal segments. Each seal segment includes a peripheral edge and a seam edge. At least a first seal segment and a second seal segment are connected along their respective peripheral edges to form a first seal layer having a seam defined by the seam edge of the first seal segment and the seam edge of the second seal segment. At least a third seal segment and a fourth seal segment are connected along their respective peripheral edges to form a second seal layer having a seam defined by the seam edge of the third seal segment and the seam edge of the fourth seal segment. The seam of the first seal layer has a first longitudinal axis and the seam of the second seal layer has a second longitudinal axis, and the first seal layer is oriented relative to the second seal layer such that the first longitudinal axis is angularly oriented relative to the second longitudinal axis.