发明授权
US07547524B2 Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion
有权
包含氨基酸插入的基因工程化的吡咯并喹啉醌依赖性葡萄糖脱氢酶
- 专利标题： Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion
- 专利标题（中）： 包含氨基酸插入的基因工程化的吡咯并喹啉醌依赖性葡萄糖脱氢酶
-
申请号： US11632672申请日： 2005-07-19
-
公开(公告)号： US07547524B2公开(公告)日： 2009-06-16
- 发明人: Mara Boenitz-Dulat , Jessica Laggerbauer , Rainer Schmuck , Peter Kratzsch , Wolfgang-Reinhold Knappe
- 申请人： Mara Boenitz-Dulat , Jessica Laggerbauer , Rainer Schmuck , Peter Kratzsch , Wolfgang-Reinhold Knappe
- 申请人地址： US IN Indianapolis
- 专利权人： Roche Diagnostics Operations, Inc.
- 当前专利权人： Roche Diagnostics Operations, Inc.
- 当前专利权人地址： US IN Indianapolis
- 代理机构： Barnes & Thornburg LLP
- 优先权： EP04017069 20040720
- 国际申请： PCT/EP2005/007844 WO 20050719
- 国际公布： WO2006/008132 WO 20060126
- 主分类号： C12Q1/32
- IPC分类号： C12Q1/32 ; C12N9/02
摘要：
The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
公开/授权文献
信息查询
