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1.发明申请Genetically Engineered Pyrroloquinoline Quinone Dependent Glucose Dehydrogenase Comprising an Amino Acid Insertion 有权基因工程化的吡咯并喹啉醌类依赖型葡萄糖脱氢酶，包括氨基酸插入公开(公告)号：US20070243566A1
公开(公告)日：2007-10-18
申请号：US11632672
申请日：2005-07-19
申请人： Mara Boenitz-Dulat , Jessica Laggerbauer , Rainer Schmuck , Peter Kratzsch , Wolfgang-Reinhold Knappe
发明人： Mara Boenitz-Dulat , Jessica Laggerbauer , Rainer Schmuck , Peter Kratzsch , Wolfgang-Reinhold Knappe
CPC分类号： C12N9/0006
摘要： The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
摘要翻译： 本发明涉及可溶性吡咯并喹啉醌（PQQ） - 依赖性葡萄糖脱氢酶（s-GDH）的改进变体，其包含对应于不动杆菌不锈棒杆菌所知的氨基酸序列的428和429位之间的氨基酸插入， s-GDH对具有改善的对葡萄糖的底物特异性的这种s-GDH变体的蛋白质以及这些s-GDH变体的不同应用，特别是用于测定样品中糖，特别是葡萄糖的浓度。
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2.发明授权Mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase 有权吡咯喹啉醌依赖性可溶性葡萄糖脱氢酶的突变体公开(公告)号：US07732179B2
公开(公告)日：2010-06-08
申请号：US12250241
申请日：2008-10-13
CPC分类号： C12N9/0006
摘要： A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
摘要翻译： 与麦芽糖相比，PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC 1.1.5.2）的突变体具有改善的葡萄糖特异性，其具有通过甘氨酸，丙氨酸或丝氨酸取代位置348处的苏氨酸，其中所述突变体 还包含至少一个用于提高突变体的稳定性的突变和用于提高突变体对葡萄糖的亲和力的一个或多个突变，和/或用于进一步提高突变体的特异性的一个或多个突变 用于与麦芽糖相比的葡萄糖，并且其中位置348对应于从A.calcoaceticus s-GDH野生型序列已知的氨基酸位置。 还公开了编码这种突变体s-GDH的基因以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。
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3.发明授权Thermostable mutants of pyrroloquinoline quinone dependent glucose dehydrogenase 有权吡咯并喹啉醌依赖性葡萄糖脱氢酶的热稳定突变体公开(公告)号：US07781196B2
公开(公告)日：2010-08-24
申请号：US11665437
申请日：2005-10-14
CPC分类号： C12N9/0006
摘要： The present invention relates to a mutant protein of PQQ-dependent s-GDH characterized in that in at least one of the positions 122 and 124 the amino acid lysine is present, wherein these positions correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence (SEQ ID NO: 2), it also discloses genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
摘要翻译： 本发明涉及PQQ依赖性s-GDH的突变蛋白，其特征在于在位置122和124中的至少一个位置存在氨基酸赖氨酸，其中这些位置对应于来自A.calcoacetus的已知氨基酸位置 s-GDH野生型序列（SEQ ID NO：2），还公开了编码这种突变体s-GDH的基因，以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。
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4.发明申请Thermostable mutants of pyrroloquinoline quinone dependent glucose dehydrogenase 有权吡咯并喹啉醌依赖性葡萄糖脱氢酶的热稳定突变体公开(公告)号：US20090305316A1
公开(公告)日：2009-12-10
申请号：US11665437
申请日：2005-10-14
CPC分类号： C12N9/0006
摘要： The present invention relates to a mutant protein of PQQ-dependent s-GDH characterized in that in at least one of the positions 122 and 124 the amino acid lysine is present, wherein these positions correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence (SEQ ID NO: 2), it also discloses genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
摘要翻译： 本发明涉及PQQ依赖性s-GDH的突变蛋白，其特征在于在位置122和124中的至少一个位置存在氨基酸赖氨酸，其中这些位置对应于来自A.calcoacetus的已知氨基酸位置 s-GDH野生型序列（SEQ ID NO：2），还公开了编码这种突变体s-GDH的基因，以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。
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5.发明授权Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion 有权包含氨基酸插入的基因工程化的吡咯并喹啉醌依赖性葡萄糖脱氢酶公开(公告)号：US07547524B2
公开(公告)日：2009-06-16
申请号：US11632672
申请日：2005-07-19
申请人： Mara Boenitz-Dulat , Jessica Laggerbauer , Rainer Schmuck , Peter Kratzsch , Wolfgang-Reinhold Knappe
发明人： Mara Boenitz-Dulat , Jessica Laggerbauer , Rainer Schmuck , Peter Kratzsch , Wolfgang-Reinhold Knappe
CPC分类号： C12N9/0006
摘要： The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
摘要翻译： 本发明涉及可溶性吡咯并喹啉醌（PQQ） - 依赖性葡萄糖脱氢酶（s-GDH）的改进变体，其包含对应于不动杆菌不锈棒杆菌所知的氨基酸序列的428和429位之间的氨基酸插入， s-GDH对具有改善的对葡萄糖的底物特异性的这种s-GDH变体的蛋白质以及这些s-GDH变体的不同应用，特别是用于测定样品中糖，特别是葡萄糖的浓度。
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6.发明申请公开(公告)号：US20090148874A1
公开(公告)日：2009-06-11
申请号：US12250241
申请日：2008-10-13
CPC分类号： C12N9/0006
摘要： A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
摘要翻译： 与麦芽糖相比，PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC 1.1.5.2）的突变体具有改善的葡萄糖特异性，其具有通过甘氨酸，丙氨酸或丝氨酸取代位置348处的苏氨酸，其中所述突变体 还包含至少一个用于提高突变体的稳定性的突变和用于提高突变体对葡萄糖的亲和力的一个或多个突变，和/或用于进一步提高突变体的特异性的一个或多个突变 用于与麦芽糖相比的葡萄糖，并且其中位置348对应于从A.calcoaceticus s-GDH野生型序列已知的氨基酸位置。 还公开了编码这种突变体s-GDH的基因以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。
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