发明授权
- 专利标题: Directed complementation
- 专利标题(中): 导向互补
-
申请号: US11398171申请日: 2006-04-05
-
公开(公告)号: US07556796B2公开(公告)日: 2009-07-07
- 发明人: Murray Robinson , Ronan O'Hagan , Karuppiah Kannan , Ti Cai , Maria Isabel Chiu , Lorena Lerner , Jie Lin , Yinghui Zhou
- 申请人: Murray Robinson , Ronan O'Hagan , Karuppiah Kannan , Ti Cai , Maria Isabel Chiu , Lorena Lerner , Jie Lin , Yinghui Zhou
- 申请人地址: US MA Cambridge
- 专利权人: AVEO Pharmaceuticals, Inc.
- 当前专利权人: AVEO Pharmaceuticals, Inc.
- 当前专利权人地址: US MA Cambridge
- 代理商 Gary L. Creason
- 主分类号: A61K49/00
- IPC分类号: A61K49/00 ; A61K35/12
摘要:
A method of producing a tumorigenic mouse cell, the tumorigenicity of which depends on a recombinant gene of interest is disclosed. The method involves: (a) providing a conditionally tumorigenic mouse cell containing a recombinant oncogene operably linked to an inducible promoter, wherein (i) expression of the recombinant oncogene is necessary and sufficient for the tumorigenicity of the tumorigenic mouse cell, and (ii) the inducible promoter is in the uninduced state; and (b) introducing into the cell a recombinant gene of interest that functionally complements the oncogene, thereby restoring tumorigenicity without expression of the inducible recombinant oncogene. Also disclosed is a method of testing a compound for anti-tumor effects. The method includes producing tumorigenic mouse cells the tumorigenicity depends on expression of a recombinant gene of interest, implanting the cells in mice and obtaining tumors from the implanted cells, administering test compounds to the mice, and determining anti-tumor effects, if any, of the compounds.
公开/授权文献
- US20060228302A1 Directed complementation 公开/授权日:2006-10-12