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    Directed complementation
    2.
    发明授权
    Directed complementation 失效
    导向互补

    公开(公告)号：US07556796B2

    公开(公告)日：2009-07-07

    申请号：US11398171

    申请日：2006-04-05

    申请人： Murray Robinson ,  Ronan O'Hagan ,  Karuppiah Kannan ,  Ti Cai ,  Maria Isabel Chiu ,  Lorena Lerner ,  Jie Lin ,  Yinghui Zhou

    发明人： Murray Robinson ,  Ronan O'Hagan ,  Karuppiah Kannan ,  Ti Cai ,  Maria Isabel Chiu ,  Lorena Lerner ,  Jie Lin ,  Yinghui Zhou

    IPC分类号： A61K49/00 ,  A61K35/12

    CPC分类号： A01K67/0271 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0331 ,  C07K14/82 ,  C12N15/8509 ,  C12N2830/002

    摘要： A method of producing a tumorigenic mouse cell, the tumorigenicity of which depends on a recombinant gene of interest is disclosed. The method involves: (a) providing a conditionally tumorigenic mouse cell containing a recombinant oncogene operably linked to an inducible promoter, wherein (i) expression of the recombinant oncogene is necessary and sufficient for the tumorigenicity of the tumorigenic mouse cell, and (ii) the inducible promoter is in the uninduced state; and (b) introducing into the cell a recombinant gene of interest that functionally complements the oncogene, thereby restoring tumorigenicity without expression of the inducible recombinant oncogene. Also disclosed is a method of testing a compound for anti-tumor effects. The method includes producing tumorigenic mouse cells the tumorigenicity depends on expression of a recombinant gene of interest, implanting the cells in mice and obtaining tumors from the implanted cells, administering test compounds to the mice, and determining anti-tumor effects, if any, of the compounds.

    摘要翻译： 公开了一种致瘤性小鼠细胞的产生方法取决于感兴趣的重组基因的方法。 该方法包括：（a）提供含有可操作地连接到诱导型启动子的重组癌基因的条件致瘤小鼠细胞，其中（i）重组癌基因的表达对于致瘤性小鼠细胞的致瘤性是必要和足够的，以及（ii） 诱导型启动子处于未诱导状态; 和（b）在细胞中引入功能上补充致癌基因的重组基因，从而恢复致瘤性而不表达可诱导的重组癌基因。 还公开了测试化合物抗肿瘤作用的方法。 该方法包括产生致瘤性小鼠细胞，其致瘤性取决于感兴趣的重组基因的表达，将细胞植入小鼠中并从植入的细胞中获得肿瘤，向小鼠施用测试化合物，并测定抗肿瘤作用（如果有的话） 化合物。

    DIRECTED COMPLEMENTATION WITH REMOVABLE GENE OF INTEREST
    4.
    发明申请
    DIRECTED COMPLEMENTATION WITH REMOVABLE GENE OF INTEREST 审中-公开
    与可移除的基因的指导性补充

    公开(公告)号：US20090165150A1

    公开(公告)日：2009-06-25

    申请号：US11963358

    申请日：2007-12-21

    申请人： Yinghui Zhou ,  William Rideout, III ,  Joerg Heyer ,  Murray Robinson

    发明人： Yinghui Zhou ,  William Rideout, III ,  Joerg Heyer ,  Murray Robinson

    IPC分类号： A01K67/027 ,  C12N15/87

    CPC分类号： C12N15/85 ,  A01K67/0271 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0331 ,  C07K14/82 ,  C12N15/8509 ,  C12N2799/027 ,  G01N2500/10

    摘要： The invention provides an improved directed complementation method for generating a conditionally tumorigenic mouse cell. In a directed complementation method, the tumorigenicity of a conditionally tumorigenic mouse cell depends on either the expression of an inducible recombinant oncogene or the expression of a recombinant gene of interest that functionally complements an uninduced recombinant oncogene. The invention provides a method of producing a tumorigenic mouse cell containing an uninduced oncogene, a recombinant gene of interest that functionally complements the uninduced oncogene, and a Cre-ER system capable of excising the recombinant gene of interest. When the Cre-ER system is activated, the recombinant gene of interest is excised. From the effect on the mouse cell it is possible to determine whether the recombinant gene of interest is a tumor maintenance gene.

    摘要翻译： 本发明提供了一种用于产生有条件致瘤性小鼠细胞的改进的定向互补方法。 在定向互补方法中，条件致瘤小鼠细胞的致瘤性取决于可诱导的重组癌基因的表达或功能上补充未诱导的重组癌基因的重组感兴趣的基因的表达。 本发明提供了一种产生致瘤性小鼠细胞的方法，所述致瘤性小鼠细胞含有未诱导的癌基因，功能上补充未诱导的致癌基因的重组基因，以及能够切除感兴趣的重组基因的Cre-ER系统。 当Cre-ER系统被激活时，切除感兴趣的重组基因。 从对小鼠细胞的作用可以确定感兴趣的重组基因是否是肿瘤维持基因。