Invention Grant
- Patent Title: Methods for producing members of specific binding pairs
- Patent Title (中): 生产特异性结合对成员的方法
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Application No.: US10803653Application Date: 2004-03-18
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Publication No.: US07662557B2Publication Date: 2010-02-16
- Inventor: John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
- Applicant: John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
- Applicant Address: GB London GB Cambridge
- Assignee: Medical Research Council,Medimmune Limited
- Current Assignee: Medical Research Council,Medimmune Limited
- Current Assignee Address: GB London GB Cambridge
- Agency: Howrey LLP
- Priority: GB9015198.6 19900710; GB9022845.3 19901019; GB9024503.6 19901112; GB9104744.9 19910306; GB9110549.4 19910515
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N33/53 ; C12P21/04 ; C40B30/04 ; C40B50/06

Abstract:
A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA.
Public/Granted literature
- US20040157215A1 Methods for producing members of specific binding pairs Public/Granted day:2004-08-12
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