发明授权
- 专利标题: Method of heat-stabilizing α-glucan phosphorylase (GP)
- 专利标题(中): 热稳定α-葡聚糖磷酸化酶(GP)的方法
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申请号: US12465135申请日: 2009-05-13
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公开(公告)号: US07723090B2公开(公告)日: 2010-05-25
- 发明人: Michiyo Yanase , Hiroki Takata , Kazutoshi Fujii , Takeshi Takaha , Takashi Kuriki
- 申请人: Michiyo Yanase , Hiroki Takata , Kazutoshi Fujii , Takeshi Takaha , Takashi Kuriki
- 申请人地址: JP Osaka
- 专利权人: Ezaki Glico Co., Ltd.
- 当前专利权人: Ezaki Glico Co., Ltd.
- 当前专利权人地址: JP Osaka
- 代理机构: Renner, Otto, Boisselle & Sklar, LLP
- 优先权: JP2003-173972 20030618
- 主分类号: C07H21/04
- IPC分类号: C07H21/04 ; C12N9/12
摘要:
An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.