利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

24 条记录 (耗时 0.036 秒)

    Method for improving the thermostability of sucrose phosphorylase (SP)
    2.
    发明授权
    Method for improving the thermostability of sucrose phosphorylase (SP) 有权
    提高蔗糖磷酸化酶（SP）热稳定性的方法

    公开(公告)号：US07968309B2

    公开(公告)日：2011-06-28

    申请号：US10566224

    申请日：2004-08-31

    申请人： Kazutoshi Fujii ,  Masae Iiboshi ,  Michiyo Yanase ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

    发明人： Kazutoshi Fujii ,  Masae Iiboshi ,  Michiyo Yanase ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

    IPC分类号： C12Q1/48 ,  C12N9/12

    CPC分类号： C12N9/1051

    摘要： A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C. for 20 minutes, is 20% or more of enzyme activity of the SP having improved thermostability at 37° C. before heating.

    摘要翻译： 提供了通过改善天然SP获得的具有改进的热稳定性的蔗糖磷酸化酶（SP）和具有改善的热稳定性的制备SP的方法。 具有改善的热稳定性的SP具有与天然蔗糖磷酸化酶不同的氨基酸残基，在选自对应于位置14的位置，对应于位置29的位置和对应于 基序序列1中的位置44; 对应于位置7的位置，对应于位置19的位置，对应于位置23的位置和对应于图案序列2中的位置34的位置; 和与基序序列3中的位置19相对应的位置，并且其中在55℃下加热在20mM Tris缓冲液（pH7.0）中具有改善的热稳定性的SP后，SP的酶活性在37℃下具有改善的热稳定性。 20分钟，在加热前在37℃下具有改善的热稳定性的SP的酶活性的20％或更多。

    METHOD OF HEAT-STABILIZING alpha-GLUCAN PHOSPHORYLASE (GP)
    3.
    发明申请
    METHOD OF HEAT-STABILIZING alpha-GLUCAN PHOSPHORYLASE (GP) 有权
    热稳定α葡萄糖磷酸酶（GP）的方法

    公开(公告)号：US20100047891A1

    公开(公告)日：2010-02-25

    申请号：US12465135

    申请日：2009-05-13

    申请人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

    发明人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

    IPC分类号： C12N9/10

    CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/04

    摘要： An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.

    摘要翻译： 提供通过改性天然α-葡聚糖磷酸化酶获得的具有改进的热稳定性的α-葡聚糖磷酸化酶，以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物，具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基，所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4，对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置，并且其中具有α-葡聚糖磷酸化酶的酶活性 在60℃下在20mM柠檬酸盐缓冲液（pH7.7）中加热10分钟后，在37℃下改善的热稳定性为具有改善的热稳定性在37℃的α-葡聚糖磷酸化酶的酶活性的20％或更多 C.加热前。

    Method of heat-stabilizing α-glucan phosphorylase(GP)
    4.
    发明授权
    Method of heat-stabilizing α-glucan phosphorylase(GP) 失效
    热稳定α-葡聚糖磷酸化酶（GP）的方法

    公开(公告)号：US07569377B2

    公开(公告)日：2009-08-04

    申请号：US10560491

    申请日：2004-06-15

    申请人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

    发明人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

    IPC分类号： C12N9/12 ,  C07H21/04

    CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/04

    摘要： An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.

    摘要翻译： 提供了通过改良天然α-葡聚糖磷酸化酶获得的具有改善的热稳定性的α-葡聚糖磷酸化酶，以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物，具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基，所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4，对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置，并且其中具有 在60℃下在20mM柠檬酸盐缓冲液（pH7.7）中加热10分钟后，在37℃下改善的热稳定性是在37℃下具有改善的热稳定性的α-葡聚糖磷酸化酶的酶活性的20％或更多 C.加热前。

    Production method and preparation method of glucans
    8.
    发明授权
    Production method and preparation method of glucans 有权
    葡聚糖的制备方法和制备方法

    公开(公告)号：US07229801B2

    公开(公告)日：2007-06-12

    申请号：US10475943

    申请日：2002-05-27

    申请人： Kazutoshi Fujii ,  Yoshinobu Terada ,  Michiyo Yanase ,  Koji Odan ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki ,  Shigetaka Okada

    发明人： Kazutoshi Fujii ,  Yoshinobu Terada ,  Michiyo Yanase ,  Koji Odan ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki ,  Shigetaka Okada

    IPC分类号： C12P19/60 ,  C12N9/10

    CPC分类号： C08B37/0009 ,  C08B30/20 ,  C08B33/00 ,  C12P19/18

    摘要： A first method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The maximum value of the sucrose-phosphate ratio of the reaction solution from the start of the reaction to the end of the reaction is no more than about 17. A second method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The reaction is conducted at a temperature of about 40 C to about 70 C.

    摘要翻译： 制备葡聚糖的第一种方法包括使含有蔗糖，底漆，无机磷酸盐或葡萄糖-1-磷酸，蔗糖磷酸化酶和葡聚糖磷酸化酶的反应溶液反应生成葡聚糖的步骤。 从反应开始到反应结束的反应溶液的蔗糖磷酸酯比例的最大值不超过约17。第二种葡聚糖的制备方法包括以下步骤：使含有蔗糖的反应溶液， 底物，无机磷酸盐或葡萄糖-1-磷酸盐，蔗糖磷酸化酶和葡聚糖磷酸化酶反应产生葡聚糖。 反应在约40℃至约70℃的温度下进行。

    Molded object comprising α-1,4-glucans and/or modifications thereof and process for producing the same
    10.
    发明授权
    Molded object comprising α-1,4-glucans and/or modifications thereof and process for producing the same 有权
    包含α-1,4-葡聚糖和/或其修饰物的成型物体及其制备方法

    公开(公告)号：US08013148B2

    公开(公告)日：2011-09-06

    申请号：US10563595

    申请日：2004-07-09

    申请人： Takeshi Takaha ,  Kazutoshi Fujii ,  Takashi Kuriki ,  Kenichi Kudo ,  Mamoru Wada ,  Michihiro Sunako ,  Junichi Takahara

    发明人： Takeshi Takaha ,  Kazutoshi Fujii ,  Takashi Kuriki ,  Kenichi Kudo ,  Mamoru Wada ,  Michihiro Sunako ,  Junichi Takahara

    IPC分类号： C07H1/00 ,  C08B30/20

    CPC分类号： C08L5/00 ,  D01F9/00 ,  Y10T428/2978

    摘要： The present application discloses a molded article, and a process for preparing a molded article consisting essentially of (i) high molecular weight linear α-1,4-glucan and (ii) low molecular weight linear α-1,4-glucan, wherein the process comprises the step of: adding the low molecular weight linear α-1,4-glucan to a solution comprising the high molecular weight linear α-1,4-glucan to gel the solution, wherein the low molecular weight linear α-1,4-glucan has a degree of polymerization of greater than or equal to 180 and less than 620, and has a molecular weight distribution of not greater than 1.25 and, the high molecular weight linear α-1,4-glucan has a degree of polymerization of greater than or equal to 620 and less than 37000, and has a molecular weight distribution of not greater than 1.25.

    摘要翻译： 本申请公开了一种模制品，以及制备基本上由（i）高分子量线性α-1,4-葡聚糖和（ii）低分子量线性α-1,4-葡聚糖组成的模制品的方法，其中 该方法包括以下步骤：将低分子量线性α-1,4-葡聚糖加入到包含高分子量线性α-1,4-葡聚糖的溶液中以使溶液凝胶化，其中低分子量线性α-1 ，4-葡聚糖具有大于或等于180且小于620的聚合度，并且分子量分布不大于1.25，高分子量线性α-1,4-葡聚糖的度 聚合度大于或等于620且小于37000，并且分子量分布不大于1.25。