发明授权
US07741089B2 Laccases, nucleic acids encoding them and methods for making and using them
有权
漆酶,编码它们的核酸和制备和使用它们的方法
- 专利标题: Laccases, nucleic acids encoding them and methods for making and using them
- 专利标题(中): 漆酶,编码它们的核酸和制备和使用它们的方法
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申请号: US10567536申请日: 2004-08-11
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公开(公告)号: US07741089B2公开(公告)日: 2010-06-22
- 发明人: Tim Hitchman , Dan E. Robertson , Masao Hiraiwa , Yoko Phillips , Kevin A. Gray
- 申请人: Tim Hitchman , Dan E. Robertson , Masao Hiraiwa , Yoko Phillips , Kevin A. Gray
- 申请人地址: US CA San Diego
- 专利权人: Verenium Corporation
- 当前专利权人: Verenium Corporation
- 当前专利权人地址: US CA San Diego
- 代理商 Jennifer L. Risser
- 国际申请: PCT/US2004/025932 WO 20040811
- 国际公布: WO2005/021714 WO 20050310
- 主分类号: C12N9/02
- IPC分类号: C12N9/02 ; C12N15/00 ; C12N1/20 ; C12N1/00 ; C12P21/06 ; C12P19/34 ; C12Q1/68 ; C07H21/04
摘要:
The invention provides laccases, polynucleotides encoding these enzymes, the use of such polynucleotides and polypeptides. In one aspect, the invention relates to the enzymatic production of nootkatone by way of the conversion of valencene using proteins having a laccase activity, e.g., a novel laccase of the invention. In one aspect, the invention provides methods of depolymerizing lignin, e.g., in a pulp or paper manufacturing process, using a polypeptide of the invention. In another aspect, the invention provides methods for oxidizing products that can be mediators of laccase-catalyzed oxidation reactions, e.g., 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 1-hydroxybenzotriazole (HBT), 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), dimethoxyphenol, and the like.
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