发明授权
US07776535B2 Site-specific incorporation of fluorinated amino acids into proteins
有权
将氟化氨基酸的位点特异性掺入蛋白质
- 专利标题: Site-specific incorporation of fluorinated amino acids into proteins
- 专利标题(中): 将氟化氨基酸的位点特异性掺入蛋白质
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申请号: US11671036申请日: 2007-02-05
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公开(公告)号: US07776535B2公开(公告)日: 2010-08-17
- 发明人: Ryan A. Mehl
- 申请人: Ryan A. Mehl
- 申请人地址: US PA Lancaster
- 专利权人: Franklin and Marshall College
- 当前专利权人: Franklin and Marshall College
- 当前专利权人地址: US PA Lancaster
- 代理机构: McNees Wallace & Nurick LLC
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34 ; C12N9/22
摘要:
This invention relates, in part, to newly identified polynucleotides, polypeptides, variants and derivatives thereof; processes for making the polynucleotides and the polypeptides, and their variants and derivatives; and uses of the polynucleotides, polypeptides, variants and derivatives. The invention also relates to compositions of orthogonal aminoacyl-tRNA synthetases, and pairs of orthogonal aminoacyl-tRNA synthetases, and orthogonal tRNAs that incorporate fluorinated amino acids into proteins in response to selector codons. The present invention also includes translation biochemistry methods for site-specific incorporation of fluorinated amino acids, for example, 18F- or 19F-labelled amino acids, into proteins or peptides. Such amino acids may be used as an NMR probe for characterizing protein structure, dynamics, and reactivity or for radionuclide imaging (e.g., PET). Fluorinated amino acids may also be used to stabilize proteins or peptides.