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    Site-specific incorporation of fluorinated amino acids into proteins
    3.
    发明授权
    Site-specific incorporation of fluorinated amino acids into proteins 有权
    将氟化氨基酸的位点特异性掺入蛋白质

    公开(公告)号：US07776535B2

    公开(公告)日：2010-08-17

    申请号：US11671036

    申请日：2007-02-05

    申请人： Ryan A. Mehl

    发明人： Ryan A. Mehl

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/22

    CPC分类号： C12P21/02 ,  C07C229/36 ,  C07K1/006 ,  C12N9/93

    摘要： This invention relates, in part, to newly identified polynucleotides, polypeptides, variants and derivatives thereof; processes for making the polynucleotides and the polypeptides, and their variants and derivatives; and uses of the polynucleotides, polypeptides, variants and derivatives. The invention also relates to compositions of orthogonal aminoacyl-tRNA synthetases, and pairs of orthogonal aminoacyl-tRNA synthetases, and orthogonal tRNAs that incorporate fluorinated amino acids into proteins in response to selector codons. The present invention also includes translation biochemistry methods for site-specific incorporation of fluorinated amino acids, for example, 18F- or 19F-labelled amino acids, into proteins or peptides. Such amino acids may be used as an NMR probe for characterizing protein structure, dynamics, and reactivity or for radionuclide imaging (e.g., PET). Fluorinated amino acids may also be used to stabilize proteins or peptides.

    摘要翻译： 本发明部分地涉及新鉴定的多核苷酸，多肽，变体和衍生物; 制备多核苷酸和多肽的方法及其变体和衍生物; 以及多核苷酸，多肽，变体和衍生物的用途。 本发明还涉及正交氨酰-tRNA合成酶的组合物，以及正交氨基-tRNA合成酶对，以及响应于选择密码子将氟化氨基酸掺入蛋白质的正交tRNA。 本发明还包括将氟化氨基酸（例如18F-或19F-标记的氨基酸）的位点特异性引入的蛋白质或肽的翻译生物化学方法。 这样的氨基酸可以用作用于表征蛋白质结构，动力学和反应性或用于放射性核素成像（例如PET）的NMR探针。 氟化氨基酸也可用于稳定蛋白质或肽。