Invention Grant
- Patent Title: Low density spreading methods for conifer somatic embryogenesis
- Patent Title (中): 针叶树体细胞胚胎发生的低密度扩散方法
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Application No.: US11856570Application Date: 2007-09-17
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Publication No.: US07785884B2Publication Date: 2010-08-31
- Inventor: James A Grob , Stephanie A Brusig
- Applicant: James A Grob , Stephanie A Brusig
- Applicant Address: US WA Federal Way
- Assignee: Weyerhaeuser NR Company
- Current Assignee: Weyerhaeuser NR Company
- Current Assignee Address: US WA Federal Way
- Agency: Christensen, O'Connor Johnson, Kindness PLLC
- Main IPC: C12N5/00
- IPC: C12N5/00

Abstract:
In one aspect, the present invention provides methods of producing conifer cotyledonary somatic embryos from pre-cotyledonary embryos. The methods of this aspect of the invention include the step of (a) dispensing a plurality of pre-cotyledonary embryos onto a porous material horizontally disposed over a non-porous surface in a volume of sterile dilution medium sufficient to submerge at least the surface of the porous material, thereby uniformly dispersing the pre-cotyledonary embryos; (b) removing the sterile dilution medium from the non-absorbent porous material, thereby trapping the uniformly dispersed pre-cotyledonary embryos on the porous material; and (c) contacting the pre-cotyledonary embryos trapped on the porous material with development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos.
Public/Granted literature
- US20080081371A1 Low Density Spreading Methods For Somatic Embryogenesis Public/Granted day:2008-04-03
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