公开(公告)号：US07785884B2

公开(公告)日：2010-08-31

申请号：US11856570

申请日：2007-09-17

Applicant: James A Grob ,  Stephanie A Brusig

Inventor： James A Grob ,  Stephanie A Brusig

IPC: C12N5/00

CPC classification number: A01H4/001 ,  A01H4/005 ,  C12N1/02

Abstract: In one aspect, the present invention provides methods of producing conifer cotyledonary somatic embryos from pre-cotyledonary embryos. The methods of this aspect of the invention include the step of (a) dispensing a plurality of pre-cotyledonary embryos onto a porous material horizontally disposed over a non-porous surface in a volume of sterile dilution medium sufficient to submerge at least the surface of the porous material, thereby uniformly dispersing the pre-cotyledonary embryos; (b) removing the sterile dilution medium from the non-absorbent porous material, thereby trapping the uniformly dispersed pre-cotyledonary embryos on the porous material; and (c) contacting the pre-cotyledonary embryos trapped on the porous material with development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos.

Abstract translation: 一方面，本发明提供了从子叶前胚胎产生针叶子子叶体细胞胚的方法。 本发明的这个方面的方法包括以下步骤：（a）将多个子叶前胚胎分配到水平设置在无菌表面上的多孔材料的无菌稀释培养基的体积中，足以浸没至少 多孔材料，从而均匀地分散子叶前胚; （b）从非吸收性多孔材料中除去无菌稀释介质，从而将均匀分散的子叶前胚胎捕获在多孔材料上; 和（c）将捕获在多孔材料上的前子叶胚胎与显影培养基接触足以产生针叶子子叶体细胞胚的时间。

公开(公告)号：US07964404B2

公开(公告)日：2011-06-21

申请号：US12164291

申请日：2008-06-30

Applicant: Roger Timmis ,  James A Grob ,  Pramod K Gupta ,  Susan D Rayfield

Inventor： Roger Timmis ,  James A Grob ,  Pramod K Gupta ,  Susan D Rayfield

IPC: C12N5/04 ,  C12N5/02 ,  A01H7/00

CPC classification number: A01H1/04 ,  A01C1/02 ,  A01H3/00 ,  A01H3/04 ,  A01H4/001 ,  A01H4/008

Abstract: In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.

Abstract translation: 在一个方面，提供了一种用于增加在体外产生的针叶树体细胞胚的发芽势的方法。 该方法包括：（a）在第一显影培养基中的第一次孵育期后，从第一胚胎培养物中分离多个单个未成熟针叶体细胞胚; 和（b）使多个单切的未成熟针叶树体细胞胚与第二培养基接触第二潜伏期。