发明授权
- 专利标题： Method for the recombination of genetic elements
- 专利标题（中）： 遗传因子重组的方法
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申请号： US10374366申请日： 2003-02-26
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公开(公告)号： US07879582B2公开(公告)日： 2011-02-01
- 发明人: Joseph Milano , Xiao-Song Tang
- 申请人： Joseph Milano , Xiao-Song Tang
- 申请人地址： US DE Wilmington
- 专利权人： E. I. du Pont de Nemours and Company
- 当前专利权人： E. I. du Pont de Nemours and Company
- 当前专利权人地址： US DE Wilmington
- 主分类号： C12N15/64
- IPC分类号： C12N15/64 ; C12N15/65 ; C12N15/00 ; C12N15/11 ; C12N15/12
摘要：
A method for the recombination of a gene is disclosed. The method involves the design of unpaired forward and reverse primers having homology to the 5′ end of one template and to the 3′ end of another template. Short primer extension periods results in a recombined template having paired 5′ and 3′ ends that can then be amplified. The amplified sample is devoid of any parental template.
公开/授权文献
- US20040014085A1 Method for the recombination of genetic elements 公开/授权日：2004-01-22